针对腐生葡萄球菌尿路致病性分离株的三种新型溶菌性噬菌体的分离、特性鉴定及基因组分析。

Isolation, characterization, and genomic analysis of three novel Herelleviridae family lytic bacteriophages against uropathogenic isolates of Staphylococcus saprophyticus.

作者信息

Shirzad-Aski Hesamaddin, Yazdi Mahsa, Mohebbi Alireza, Rafiee Maryam, Soleimani-Delfan Abbas, Tabarraei Alijan, Ghaemi Ezzat Allah

机构信息

Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran.

Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, 49341-74515, Iran.

出版信息

Virol J. 2025 Mar 27;22(1):87. doi: 10.1186/s12985-025-02710-0.

DOI:10.1186/s12985-025-02710-0

PMID:40148969

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11951804/

Abstract

BACKGROUND

Staphylococcus saprophyticus (S. saprophyticus) is the second most prevalent etiological agent of urinary tract infections (UTIs) in young women. However, there is a paucity of data regarding its bacteriophage (phage). Therefore, this study was conducted to isolate and identify new lytic phages from municipal wastewater with the objective of increasing knowledge about phages and their genomes.

METHODS

A total of 11 clinical isolates of S. saprophyticus and 30 wastewater samples were used to isolate three lytic phages (vB_SsapH-Golestan-100, vB_SsapH-Golestan101-M, and vB_SsapH-Golestan-105-M). The morphology, behavioral characteristics, and complete DNA genomes of these phages were analyzed.

RESULTS

The microscopic images of the phages revealed that the sizes of their heads and tail lengths fell within the ranges of 90-111 nm and 234-266 nm, respectively. All phages exhibited high adsorption rates (99.5% in 15 min) and burst sizes (150-210 PFU per infected cell), with a potential for a narrow host range. Genomic analysis of Staphylococcus phages indicated a size of 136,433 base pairs (bp) with a guanine-cytosine (GC) content of 33.7% and 192 open reading frames (ORFs) for vB_SsapH-Golestan-100, 144,081 bp with a GC content of 29.6% and 205 ORFs for vB_SsapH-Golestan101-M, and 142,199 bp with a GC content of 30.6% and 203 ORFs for vB_SsapH-Golestan-105-M. A bioinformatics analysis indicated that all three phages belong to the Twortvirinae subfamily of Herelleviridae. Among the three phages, vB_SsapH-Golestan-100 exhibited the least similarity to previously known phages, with less than 21% similarity with its closest counterparts in genomic databases.

CONCLUSIONS

This study identified new phages that have the ability to destroy a broad range of S. saprophyticus isolates and may potentially be classified as a new genus and species within the Herelleviridae family in future studies.

摘要

背景

腐生葡萄球菌是年轻女性尿路感染（UTIs）的第二大常见病原体。然而，关于其噬菌体的数据却很少。因此，本研究旨在从城市废水中分离和鉴定新型裂解性噬菌体，以增加对噬菌体及其基因组的了解。

方法

共使用11株腐生葡萄球菌临床分离株和30份废水样本分离出三株裂解性噬菌体（vB_SsapH-Golestan-100、vB_SsapH-Golestan101-M和vB_SsapH-Golestan-105-M）。对这些噬菌体的形态、行为特征和完整DNA基因组进行了分析。

结果

噬菌体的显微镜图像显示，其头部大小和尾部长度分别在90-111nm和234-266nm范围内。所有噬菌体均表现出高吸附率（15分钟内达99.5%）和裂解量（每个感染细胞产生150-210个噬菌斑形成单位），宿主范围可能较窄。葡萄球菌噬菌体的基因组分析表明，vB_SsapH-Golestan-100的基因组大小为136,433碱基对（bp），鸟嘌呤-胞嘧啶（GC）含量为33.7%，有192个开放阅读框（ORF）；vB_SsapH-Golestan101-M的基因组大小为144,081bp，GC含量为29.6%，有205个ORF；vB_SsapH-Golestan-105-M的基因组大小为142,199bp，GC含量为30.6%，有203个ORF。生物信息学分析表明，这三株噬菌体均属于赫勒病毒科的Twortvirinae亚科。在这三株噬菌体中，vB_SsapH-Golestan-100与先前已知噬菌体的相似性最低，在基因组数据库中与其最接近的对应物的相似性不到21%。