Pérez Lourdes, da Silva Cecília Rocha, do Amaral Valente Sá Lívia Gurgel, Neto João Batista de Andrade, Cabral Vitória Pessoa de Farias, Rodrigues Daniel Sampaio, Moreira Lara Elloyse Almeida, Silveira Maria Janielly Castelo Branco, Ferreira Thais Lima, da Silva Anderson Ramos, Cavalcanti Bruno Coêlho, Ricardo Nágila Maria Pontes Silva, Rodrigues Francisco Alessandro Marinho, Júnior Hélio Vitoriano Nobre
Department of Surfactants and Nanobiotechnology, Instituto de Química Avanzada de Cataluña, Consejo Superior de Investigaciones Químicas (IQAC-CSIC), 08034 Barcelona, Spain.
Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), School of Pharmacy, Federal University of Ceará, Fortaleza 60020-181, Brazil.
Antibiotics (Basel). 2025 Feb 24;14(3):227. doi: 10.3390/antibiotics14030227.
Mixed bloodstream infections associated with central venous catheter (CVC) use are a growing problem. The aim of this study was to evaluate the activity of a cationic arginine-based gemini surfactant, C(LA), against mixed biofilms of fluconazole-resistant and extended-spectrum beta-lactamase (ESBL)-producing , and the preventive effect of this surfactant impregnated in CVCs on the formation of inter-kingdom biofilms. Broth microdilution assays were performed along with evaluation of the effect against mixed biofilms in formation. The impregnation of CVCs with the surfactant and with a hydrogel containing the cationic surfactant was investigated to assess their potential to prevent the formation of mixed biofilms. Scanning electron microscopy (SEM) was also utilized. Minimum inhibitory concentrations (MICs) for resistant ranged from 4-5.3 µg/mL, while for , the MICs varied from 85.3 to 298.7 µg/mL. Fungicidal and bactericidal action patterns were obtained. In mixed biofilm formation in 96-well plates, there was a significant reduction in the colony-forming unit (CFU) count. The impregnation of the CVC with C(LA) alone resulted in a biofilm reduction of 62% versus and 48.7% against in terms of CFUs. When the CVC was impregnated with the surfactant hydrogel, the effect was improved with an inhibition of 71.7% for and 86.7% for . The images obtained by SEM corroborated the results. C(LA) has potential for use in CVC impregnation to prevent the formation of mixed biofilms of fluconazole-resistant and ESBL-producing .
与中心静脉导管（CVC）使用相关的混合血流感染问题日益严重。本研究旨在评估一种基于阳离子精氨酸的双子表面活性剂C(LA)对耐氟康唑和产超广谱β-内酰胺酶（ESBL）的混合生物膜的活性，以及这种浸渍在CVC中的表面活性剂对跨界生物膜形成的预防作用。进行了肉汤微量稀释试验，并评估了对正在形成的混合生物膜的影响。研究了用表面活性剂和含阳离子表面活性剂的水凝胶浸渍CVC，以评估它们预防混合生物膜形成的潜力。还利用了扫描电子显微镜（SEM）。对耐药菌的最低抑菌浓度（MIC）范围为4-5.3μg/mL，而对产ESBL菌，MIC范围为85.3至298.7μg/mL。获得了杀菌和抑菌作用模式。在96孔板中形成混合生物膜时，菌落形成单位（CFU）计数显著减少。仅用C(LA)浸渍CVC，就CFU而言，对真菌的生物膜减少了62%，对产ESBL菌减少了48.7%。当CVC用表面活性剂水凝胶浸渍时，效果得到改善，对真菌的抑制率为71.7%，对产ESBL菌的抑制率为86.7%。通过SEM获得的图像证实了结果。C(LA)有潜力用于浸渍CVC，以防止耐氟康唑真菌和产ESBL菌的混合生物膜形成。
