Wang Zhi-Qi, Yu Tai-Fei, Sun Guo-Zhong, Zheng Jia-Cheng, Chen Jun, Zhou Yong-Bin, Chen Ming, Ma You-Zhi, Wei Wen-Liang, Xu Zhao-Shi
College of Agriculture, Yangtze University, Hubei Collaborative Innovation Center for Grain Industry, Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Jingzhou, China.
Institute of Crop Science, Chinese Academy of Agricultural Sciences(CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, China.
Front Plant Sci. 2021 Mar 18;12:614909. doi: 10.3389/fpls.2021.614909. eCollection 2021.
Abiotic stresses, such as drought and salinity, severely affects the growth, development and productivity of the plants. The RLK1-like (CrRLK1L) protein kinase family is involved in several processes in the plant life cycle. However, there have been few studies addressing the functions of CrRLK1L proteins in soybean. In this study, 38 genes were identified in the soybean genome ( Wm82.a2.v1). Phylogenetic analysis demonstrated that soybean genes were grouped into clusters, cluster I, II, III. The chromosomal mapping demonstrated that 38 genes were located in 14 of 20 soybean chromosomes. None were discovered on chromosomes 1, 4, 6, 7, 11, and 14. Gene structure analysis indicated that 73.6% soybean genes were characterized by a lack of introns.15.7% soybean genes only had one intron and 10.5% soybean genes had more than one intron. Five genes were obtained from soybean drought- and salt-induced transcriptome databases and were found to be highly up-regulated. was notably up-regulated under drought and salinity stresses, and was therefore studied further. Subcellular localization analysis revealed that the protein was located in the cell membrane. The overexpression of the gene in soybean hairy roots improved both drought tolerance and salt stresses and enhanced the expression of the stress-responsive genes , , , , , and . These results indicated that could play a vital role in defending against drought and salinity stresses in soybean.
干旱和盐度等非生物胁迫严重影响植物的生长、发育和生产力。类受体样激酶1（CrRLK1L）蛋白激酶家族参与植物生命周期的多个过程。然而，关于CrRLK1L蛋白在大豆中的功能研究较少。在本研究中，在大豆基因组（Wm82.a2.v1）中鉴定出38个基因。系统发育分析表明，大豆基因被分为I、II、III类群。染色体定位表明，38个基因位于20条大豆染色体中的14条上，在1、4、6、7、11和14号染色体上未发现。基因结构分析表明，73.6%的大豆基因无内含子，15.7%的大豆基因只有一个内含子，10.5%的大豆基因有多个内含子。从大豆干旱和盐诱导转录组数据库中获得了5个基因，发现它们高度上调。其中一个基因在干旱和盐胁迫下显著上调，因此对其进行了进一步研究。亚细胞定位分析表明，该蛋白位于细胞膜上。该基因在大豆毛状根中的过表达提高了耐旱性和耐盐性，并增强了胁迫响应基因、、、、和的表达。这些结果表明，该基因在大豆抵御干旱和盐胁迫中可能发挥重要作用。
