Guo Suqin, Wu Xiqun, Peng Feng, Zhang Kun, Sooranna Suren Rao, Tan Guiyu
School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China.
Key Laboratory of High-Quality Formation and Utilization of Dao-di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
Genes (Basel). 2025 Mar 8;16(3):321. doi: 10.3390/genes16030321.
Hook. f. and K. I. B.et K. I. M. are two important medicinal plants which grow in the mountainous areas of Guangxi, China. Their similar morphological characteristics frequently lead to their misidentification. Chloroplast genome (cp)-based barcode technology has been used to effectively identify two closely related species; however, at present, there is no systematic comparative study of the cp genome sequences between and . : Here, the cp genomes of the two plants were sequenced and analyzed. : The cp genome sizes were 142,689 and 142,689 bp for and , respectively. Each of the cp genomes annotated 122 genes, with 79 protein coding genes, 8 ribosomal RNA genes, and 35 transfer RNA genes. Amino acid frequencies of 1.17-10.19% () and 1.18-10.17% () were found in the coding genes. There were also 104 and 96 SSRs as well as 26 and 25 long repeats identified in and , respectively, among which the most common were A/T base repeats. Both cp genomes had SSC/IRa junctions located in gene The and genes were positioned at the IRb/SSC and LSC/IR boundaries, respectively. A phylogenetic relationship was constructed and the two species were fully nested within the genus . : The comparative cp genomes of and are presented in this study, and this provides valuable phylogenetic information for subsequent molecular marker development and research of and .
胡颓子属植物和鸡屎藤是两种在中国广西山区生长的重要药用植物。它们相似的形态特征常常导致它们被误认。基于叶绿体基因组（cp）的条形码技术已被用于有效识别两个近缘物种；然而，目前尚未对两者之间的cp基因组序列进行系统的比较研究。在此，对这两种植物的cp基因组进行了测序和分析。胡颓子属植物和鸡屎藤的cp基因组大小分别为142,689 bp。每个cp基因组注释了122个基因，其中包括79个蛋白质编码基因、8个核糖体RNA基因和35个转运RNA基因。在编码基因中发现的氨基酸频率分别为1.17 - 10.19%（胡颓子属植物）和1.18 - 10.17%（鸡屎藤）。在胡颓子属植物和鸡屎藤中还分别鉴定出104个和96个简单序列重复（SSR）以及26个和25个长重复序列，其中最常见的是A/T碱基重复。两个cp基因组的SSC/IRa连接点都位于基因处。胡颓子属植物和鸡屎藤的基因分别位于IRb/SSC和LSC/IR边界。构建了系统发育关系，这两个物种完全嵌套在胡颓子属内。本研究展示了胡颓子属植物和鸡屎藤的cp基因组比较，这为后续胡颓子属植物和鸡屎藤的分子标记开发和研究提供了有价值的系统发育信息。
