Cheng Chiao-Yin, Chen Yen-Lin, Ho Hua, Huang Chun-Yen, Chu Sheng-En, Liang Yao-Jen
Graduate Institute of Applied Science and Engineering, Fu-Jen Catholic University, New Taipei 242062, Taiwan.
Department of Emergency Medicine, Far Eastern Memorial Hospital, New Taipei 220216, Taiwan.
Diagnostics (Basel). 2025 Mar 7;15(6):652. doi: 10.3390/diagnostics15060652.
Gastric cancer is one of the most common and lethal cancers worldwide, with particularly high incidence and mortality rates in East Asia and Europe. DNAJB4 has been shown to have prognostic implications in other cancer types; however, its expression patterns and role in gastric cancer have not been extensively studied. This study aimed to analyze DNAJB4 expression in gastric cancer and explore its association with clinical characteristics, molecular markers, and patient outcomes. We selected suitable tumor samples from 189 gastric cancer patients who had not undergone chemotherapy or radiotherapy, with 188 patients ultimately included in the analysis. Tissue microarray and immunohistochemistry were used to evaluate DNAJB4 expression, and the samples were divided into high- and low-expression groups based on the H-score. Multivariate logistic regression and survival analysis were conducted to identify influencing factors. High DNAJB4 expression was significantly correlated with increased CD31 levels but was inversely associated with advanced cancer stages. Subgroup analysis revealed that in patients with advanced gastric cancer, high DNAJB4 expression was associated with increased caspase-3 levels and with elevated CD31 and decreased E-cadherin levels. High DNAJB4 expression was associated with both angiogenesis and apoptosis, indicating its complex role in gastric cancer progression. Although DNAJB4 promoted angiogenesis by increasing CD31 levels, it may also enhance apoptosis in tumor cells through caspase-3-induced apoptosis.
胃癌是全球最常见且致命的癌症之一，在东亚和欧洲的发病率和死亡率尤其高。DNAJB4已被证明在其他癌症类型中具有预后意义；然而，其在胃癌中的表达模式和作用尚未得到广泛研究。本研究旨在分析DNAJB4在胃癌中的表达，并探讨其与临床特征、分子标志物及患者预后的关系。我们从189例未接受过化疗或放疗的胃癌患者中选取了合适的肿瘤样本，最终188例患者纳入分析。采用组织芯片和免疫组化评估DNAJB4表达，并根据H评分将样本分为高表达组和低表达组。进行多因素逻辑回归和生存分析以确定影响因素。DNAJB4高表达与CD31水平升高显著相关，但与癌症晚期呈负相关。亚组分析显示，在晚期胃癌患者中，DNAJB4高表达与caspase-3水平升高、CD31水平升高及E-钙黏蛋白水平降低有关。DNAJB4高表达与血管生成和凋亡均相关，表明其在胃癌进展中起复杂作用。尽管DNAJB4通过增加CD31水平促进血管生成，但它也可能通过caspase-3诱导的凋亡增强肿瘤细胞的凋亡。
