Clark John D, Baker Christopher A, McGeachy Sarah A, Jensen Rebecca R, Benfey Tillmann J
Department of Biology, University of New Brunswick, Fredericton, New Brunswick, Canada.
J Fish Biol. 2025 Jul;107(1):292-297. doi: 10.1111/jfb.70031. Epub 2025 Mar 27.
Routine observations of blood smears have repeatedly shown that triploid fish have a greater proportion of erythrocytes with nuclear segmentation (ENS) than diploids; however, there is as yet no understanding of why this is the case and whether it affects erythrocyte function. In an attempt to address this, we examined blood smears from two previous experiments to determine whether ENS frequency is affected by rearing conditions (acclimation temperature or dissolved oxygen level) and subsequent acute aerobic distress in diploid and triploid brook charr Salvelinus fontinalis (Mitchill 1814). As expected, triploids had a higher ENS frequency than corresponding diploids, but with no biologically significant effect of experimental conditions on ENS frequency in triploids. ENS was too rare to examine the effects of these conditions in diploids.
对血涂片的常规观察反复表明，三倍体鱼中具有核分割（ENS）的红细胞比例高于二倍体鱼；然而，目前尚不清楚为何会出现这种情况以及它是否会影响红细胞功能。为了解决这个问题，我们检查了之前两个实验中的血涂片，以确定ENS频率是否受养殖条件（驯化温度或溶解氧水平）以及二倍体和三倍体溪红点鲑（Salvelinus fontinalis，米契尔，1814年）随后的急性需氧应激影响。正如预期的那样，三倍体的ENS频率高于相应的二倍体，但实验条件对三倍体的ENS频率没有生物学上的显著影响。ENS非常罕见，无法在二倍体中研究这些条件的影响。
