Li Yun-Shan, Fujihara Hiroaki, Fujisawa Koichi, Kawai Kazuaki
Department of Environmental Oncology, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu-shi, Fukuoka 807-8555, Japan.
Center for Stress-related Disease Control and Prevention, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu-shi, Fukuoka 807-8555, Japan.
J Clin Biochem Nutr. 2025 Mar;76(2):117-124. doi: 10.3164/jcbn.24-220. Epub 2024 Dec 26.
Ergothioneine has antioxidant, anti-inflammatory, and cell-protective properties. Circadian rhythm disruption can lead to health issues, such as insomnia, mental illness, chronic diseases, and cancer. However, the impact of ergothioneine, as an antioxidant, on oxidative DNA damage and immune variations caused by circadian rhythm disruptions remains unclear. To investigate the effect of ergothioneine on oxidative DNA damage and immune responses caused by circadian rhythm disruption, 8-week-old mice were subjected to night time feeding and exercise restrictions for 14 days. Body weight, daytime running wheel activity, and anxiety-like behavior showed no significant differences between the night-restricted groups, regardless of ergothioneine administration. Serum interleukin-6 levels, 8-hydroxy-2'-deoxyguanosine levels in urine and nuclear DNA of the liver, testes, lungs, and pancreas were significantly reduced in the night-restricted group receiving ergothioneine compared with that of the group without ergothioneine, with no significant differences observed when compared to the control group. Ergothioneine can mitigate immune function changes and oxidative DNA damage induced by circadian rhythm disruption caused by abnormal dietary timing in mice. However, it did not alleviate obesity or mental state dysregulation. These findings have important implications for improving night-shift workers health and developing therapies for diseases associated with circadian rhythm disturbances.
麦角硫因具有抗氧化、抗炎和细胞保护特性。昼夜节律紊乱会导致健康问题，如失眠、精神疾病、慢性疾病和癌症。然而，麦角硫因作为一种抗氧化剂，对昼夜节律紊乱引起的氧化性DNA损伤和免疫变化的影响仍不清楚。为了研究麦角硫因对昼夜节律紊乱引起的氧化性DNA损伤和免疫反应的影响，对8周龄小鼠进行了14天的夜间喂食和运动限制。无论是否给予麦角硫因，夜间限制组之间的体重、白天转轮活动和焦虑样行为均无显著差异。与未给予麦角硫因的组相比，给予麦角硫因的夜间限制组血清白细胞介素-6水平、尿液以及肝脏、睾丸、肺和胰腺的核DNA中的8-羟基-2'-脱氧鸟苷水平显著降低，与对照组相比无显著差异。麦角硫因可以减轻小鼠因异常饮食时间导致的昼夜节律紊乱所引起的免疫功能变化和氧化性DNA损伤。然而，它并没有缓解肥胖或精神状态失调。这些发现对于改善夜班工作者的健康以及开发与昼夜节律紊乱相关疾病的治疗方法具有重要意义。
