Li Yun-Shan, Fujihara Hiroaki, Fujisawa Koichi, Kawai Kazuaki
Department of Environmental Oncology, University of Occupational and Environmental Health, Japan, Kitakyushu, Fukuoka 807-8555, Japan.
Center for Stress-related Disease Control and Prevention, University of Occupational and Environmental Health, Japan, Kitakyushu, Fukuoka 807-8555, Japan.
J Clin Biochem Nutr. 2025 Jan;76(1):35-41. doi: 10.3164/jcbn.24-126. Epub 2024 Aug 22.
Frequent or long-term circadian disorders can lead to a range of health problems, including chronic insomnia, depression, chronic diseases, and cancer. It has also been shown that altering the feeding time of mice from night to day can result in circadian disorder. Recent studies have revealed complex interactions between circadian rhythm and oxidative stress. However, little is known about the impact of circadian rhythm disorders caused by time-restricted feeding on mental state, immune function, and oxidative DNA damage. In this study, we investigated the effects of circadian rhythm disruption by controlling the timing of feeding and exercise on oxidative DNA damage and immune responses in 8-week-old mice for 14 days. Body weight, daytime running wheel activity, serum interleukin-6 levels, urinary 8-hydroxy-2'-deoxyguanosine levels, and nuclear DNA (liver, lung, testes, and pancreas) were significantly increased in the night-restricted group compared with the control group. Additionally, the mice in the night-restricted group exhibited anxiety-like behavior. These results indicated that the circadian rhythm disruption due to abnormal dietary timing can lead to obesity, mental state dysregulation, immune function changes and oxidative DNA damage in mice. This oxidative DNA damage may contribute to the initiation and increased risk of cancer.
频繁或长期的昼夜节律紊乱会导致一系列健康问题，包括慢性失眠、抑郁症、慢性病和癌症。研究还表明，将小鼠的进食时间从夜间改为白天会导致昼夜节律紊乱。最近的研究揭示了昼夜节律与氧化应激之间的复杂相互作用。然而，关于限时进食引起的昼夜节律紊乱对精神状态、免疫功能和氧化性DNA损伤的影响知之甚少。在本研究中，我们通过控制8周龄小鼠14天的进食和运动时间，研究了昼夜节律紊乱对氧化性DNA损伤和免疫反应的影响。与对照组相比，夜间限制组的体重、白天转轮活动、血清白细胞介素-6水平、尿8-羟基-2'-脱氧鸟苷水平和核DNA（肝脏、肺、睾丸和胰腺）显著增加。此外，夜间限制组的小鼠表现出焦虑样行为。这些结果表明，异常饮食时间导致的昼夜节律紊乱可导致小鼠肥胖、精神状态失调、免疫功能改变和氧化性DNA损伤。这种氧化性DNA损伤可能会导致癌症的发生并增加患癌风险。
