Sefah Bernard, Ashie Yolanda, Osafo Newman, Mante Priscilla Kolibea
Department of Pharmacology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Department of Pathology, Komfo Anokye Teaching Hospital, Kumasi, Ghana.
ScientificWorldJournal. 2025 Mar 20;2025:2198542. doi: 10.1155/tswj/2198542. eCollection 2025.
Neurodegenerative disorders are known to be commonly associated with neuroinflammation. Plants with antioxidant and anti-inflammatory properties hold prospect in alleviating neuroinflammation. One such plant with documented anti-inflammatory and antioxidant potential is L. This study looked at effects of the hydroethanolic leaf extract of L. on lipopolysaccharide (LPS)-induced neuroinflammation and associated memory impairment using an ICR mouse model. Assessment of the phytochemical constituents in L. and its acute toxicity was conducted. Mice were treated with L. extract (30, 100, and 300 mg/kg) after LPS administration. Object recognition and elevated plus maze tests were employed to assess neuroinflammation-induced behavioral changes. Brain samples were taken to determine the levels of TNF- and conduct histopathological analysis. The hydroethanolic extract of L. was found to contain alkaloids, glycoside, tannins, flavonoids, and coumarins and exhibited no observable acute toxicity. The extract showed the presence of eicosatrienoic acid, methyl ester, and phenanthrene derivatives. The extract improved memory and cognitive performance but had no significant effect on brain tissue TNF- expression. L. treatment in mice with neuroinflammation also resulted in reduced mononuclear infiltration and gliosis and reduced apoptotic and necrotic neurons as well as no observable brain lesions. L. holds promising pharmacological activity at reducing neuroinflammation and its associated cognitive impairment.
已知神经退行性疾病通常与神经炎症相关。具有抗氧化和抗炎特性的植物在减轻神经炎症方面具有前景。一种具有已记录的抗炎和抗氧化潜力的此类植物是L。本研究使用ICR小鼠模型研究了L的水乙醇叶提取物对脂多糖（LPS）诱导的神经炎症及相关记忆障碍的影响。对L中的植物化学成分及其急性毒性进行了评估。在给予LPS后，用L提取物（30、100和300mg/kg）处理小鼠。采用物体识别和高架十字迷宫试验来评估神经炎症诱导的行为变化。采集脑样本以测定TNF水平并进行组织病理学分析。发现L的水乙醇提取物含有生物碱、糖苷、单宁、黄酮类化合物和香豆素，且未表现出明显的急性毒性。提取物显示存在二十碳三烯酸甲酯和菲衍生物。提取物改善了记忆和认知表现，但对脑组织TNF表达无显著影响。用L处理神经炎症小鼠还导致单核细胞浸润和胶质细胞增生减少，凋亡和坏死神经元减少，且未观察到脑损伤。L在减轻神经炎症及其相关认知障碍方面具有有前景的药理活性。
