Song Meijun, Jia Bingke, Dai Dinghui, Xu Xiaoli, Cao Jiaxue, Guo Jiazhong, Wang Linjie, Zhong Tao, Zhan Siyuan, Li Li, Zhang Hongping
Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Front Vet Sci. 2025 Mar 13;12:1544234. doi: 10.3389/fvets.2025.1544234. eCollection 2025.
Optimizing buck semen preservation techniques can significantly advance the goat industry. This study aimed to investigate the effects of chitosan on sperm quality and seminal plasma metabolite profiles in bucks during low-temperature storage at 4°C.
The results showed that when 0.2 mg/mL chitosan was added to semen dilution, sperm viability and antioxidant capacity were highest and significantly higher than the control group ( < 0.05). Sperm viability decreased progressively with increasing storage time at 4°C. However, on day 5, sperm viability was significantly higher in all groups where chitosan was added to the semen dilutions than in the control group ( < 0.05). A total of 23 classes of metabolites were detected in the non-targeted metabolism group of seminal plasma. The metabolite caused by chitosan mainly included fatty acyls, phospholipids, amino acids and organic acids. Most differential metabolites in fatty acyls and glycerophospholipids in chitosan-treated semen were decreased and enriched in the anabolic pathway of unsaturated fatty acids. Additionally, several oligopeptides showed correlations with sperm quality.
These results suggest that adding 0.2 mg/mL chitosan to semen diluent successfully prolongs the low-temperature preservation of semen mainly by altering the anabolism of lipids and amino acids. This provides theoretical support and practical reference for the applying chitosan in the low-temperature preservation of buck semen.
优化公鹿精液保存技术可显著推动养羊业发展。本研究旨在探讨壳聚糖对4℃低温保存期间公鹿精子质量和精浆代谢物谱的影响。
结果表明，向精液稀释液中添加0.2mg/mL壳聚糖时，精子活力和抗氧化能力最高，且显著高于对照组（P<0.05）。在4℃下，精子活力随保存时间延长而逐渐下降。然而，在第5天，所有添加壳聚糖的精液稀释液组的精子活力均显著高于对照组（P<0.05）。在精浆非靶向代谢组中共检测到23类代谢物。壳聚糖引起的代谢物主要包括脂肪酰基、磷脂、氨基酸和有机酸。壳聚糖处理精液中脂肪酰基和甘油磷脂中的大多数差异代谢物减少，并在不饱和脂肪酸的合成代谢途径中富集。此外，几种寡肽与精子质量呈相关性。
这些结果表明，向精液稀释液中添加0.2mg/mL壳聚糖主要通过改变脂质和氨基酸的合成代谢成功延长了精液的低温保存时间。这为壳聚糖在公鹿精液低温保存中的应用提供了理论支持和实践参考。
