State Key Laboratory of Food Science and Technology, Nanchang University, Jiangxi 330047, China.
Jiangxi Academy of Sciences, Jiangxi 330029, China.
Int J Biol Macromol. 2022 Jun 15;210:53-62. doi: 10.1016/j.ijbiomac.2022.05.031. Epub 2022 May 7.
The composite films were prepared by adding rice protein hydrolysate (RH) into chitosan (CH). Effect of four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa) was evaluated and curcumin was further incorporated into the film to enhance its bioactivities. With increasing MW (<100 kDa), moisture, solubility and water vapor transmission marginally decreased while tensile strength increased. Rice hydrolysate improved the film solubility and extensibility. All films showed extraordinary extensibility ranging from 180.26% to 204.08%. DSC confirmed MW did affect the thermal stability. The lower MW, the higher antioxidant activity of the CH/RH films. The 30 kDa CH/RH film exhibited higher antibacterial activity against both E. coli and S. aureus. Curcumin effectively improved both antibacterial and antioxidant activity of the CH/RH films. Hence, chitosan molecular weight must be chosen carefully according to its application and incorporation of protein hydrolysate into film would be an efficient strategy to modify film performance.
将大米蛋白水解物（RH）添加到壳聚糖（CH）中制备复合膜。评价了四种不同的壳聚糖分子量（MW，0.8-1.0、30、100、300 kDa）的影响，并进一步将姜黄素掺入到膜中以提高其生物活性。随着 MW（<100 kDa）的增加，水分、溶解度和水蒸气透过率略有降低，而拉伸强度增加。米水解物提高了膜的溶解度和延展性。所有薄膜的延展性均非常出色，范围为 180.26%至 204.08%。DSC 证实 MW 确实影响了热稳定性。MW 越低，CH/RH 薄膜的抗氧化活性越高。30 kDa 的 CH/RH 薄膜对大肠杆菌和金黄色葡萄球菌均表现出更高的抗菌活性。姜黄素有效地提高了 CH/RH 薄膜的抗菌和抗氧化活性。因此，必须根据其应用谨慎选择壳聚糖分子量，并且将蛋白水解物掺入薄膜中是改善薄膜性能的有效策略。
