人和大鼠红细胞对51铬（VI）的体外快速摄取动力学。

Fast uptake kinetics in vitro of 51Cr (VI) by red blood cells of man and rat.

作者信息

Wiegand H J, Ottenwälder H, Bolt H M

出版信息

Arch Toxicol. 1985 Apr;57(1):31-4. doi: 10.1007/BF00286571.

Abstract

Fast transport kinetics of 51Cr (VI) into red blood cells (RBCs) in vitro were studied. No significant species differences were found between RBCs of man and rat. The uptake of 51Cr (VI) by RBCs in whole blood was composed of two different first order processes of different velocities (apparent t 1/2 of 22.7 s and 10.4 min for man and 6.9 s and 10.1 min for rat, respectively). However, even after longer time periods a fixed portion of approximately 15% of the administered dose remained in the plasma and did not penetrate into RBCs Over the entire concentration range studied (10 microM-50 mM), the fast initial uptake followed Michaelis-Menten kinetics. The maximal capacity of this Cr(VI) transport into RBCs of man and rat was 3.1 X 10(8) CrO4(2-) ions X cell-1 X min-1 and 2.5 X 10(8) CrO4(-2) ions X cell-1 X min-1, respectively. It is likely that Cr(VI) is transported into RBCs via a physiological anion carrier ("band-3-protein").

摘要

研究了体外51Cr（VI）进入红细胞（RBC）的快速转运动力学。在人和大鼠的红细胞之间未发现明显的物种差异。全血中红细胞对51Cr（VI）的摄取由两个不同速度的一级过程组成（人分别为22.7秒和10.4分钟的表观半衰期，大鼠分别为6.9秒和10.1分钟）。然而，即使在较长时间段后，仍有大约15%的给药剂量固定部分留在血浆中，未渗透到红细胞中。在所研究的整个浓度范围内（10 microM - 50 mM），快速的初始摄取遵循米氏动力学。人和大鼠的红细胞中这种Cr（VI）转运的最大容量分别为3.1×10（8）个CrO4（2-）离子×细胞-1×分钟-1和2.5×10（8）个CrO4（-2）离子×细胞-1×分钟-1。Cr（VI）可能通过生理阴离子载体（“带3蛋白”）转运到红细胞中。