Owiredu Nikki, Lean Soo Sum, Stegger Marc, Allison Hui San, Hewson Kylie, Sharpe Sue, Pavic Anthony, Lugsomya Kittitat, Jordan David, Hampson David J, Abraham Sam, Abraham Rebecca
Centre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Perth, Australia.
School of Medical, Molecular, and Forensic Sciences, Murdoch University, Perth, Australia.
Sci Rep. 2025 Mar 28;15(1):10780. doi: 10.1038/s41598-025-94453-9.
The increasing resistance of bacteria to antimicrobials is a major threat to public health. This study investigates the prevalence of antimicrobial resistance, both phenotypic and genotypic, among Campylobacter isolates from Australian meat chickens in 2022, as a follow up to investigate trends since the last national surveillance undertaken in 2016. Isolates (n = 186) were obtained at slaughter from 200 pooled cecal samples taken from 1,000 meat chickens. The majority of C. jejuni (68.7%) and C. coli (88.9%) isolates were susceptible to all the antibiotics that were tested, and no multi-drug resistance was found. Resistance to ciprofloxacin (fluoroquinolone) was detected in 24.4% of the C. jejuni and 3.2% of the C. coli isolates. Whole genome sequencing revealed a diverse range of sequence types (STs). These included 32 previously reported STs for C. jejuni and 13 for C. coli, as well as four and seven previously undescribed STs for each species, respectively. The STs containing fluoroquinolone-resistant isolates were ST2083, ST10130, ST2895, ST7323, ST2398, and ST1078 for C. jejuni, and ST860 and ST894 for C. coli. Although fluoroquinolones are not used in animal production in Australia, resistance amongst C. jejuni isolates was high (24.4%). This finding emphasizes the need for enhanced surveillance and regular sampling along the food chain to understand the source of the isolates and to mitigate risks of antimicrobial resistance to protect public health.
细菌对抗菌药物的耐药性不断增加是对公众健康的重大威胁。本研究调查了2022年澳大利亚肉鸡弯曲杆菌分离株中表型和基因型抗菌药物耐药性的流行情况，作为2016年上次全国监测以来调查趋势的后续研究。从1000只肉鸡采集的200份盲肠样本池中获取了186株分离株。大多数空肠弯曲杆菌（68.7%）和结肠弯曲杆菌（88.9%）分离株对所有测试抗生素敏感，未发现多重耐药性。在24.4%的空肠弯曲杆菌和3.2%的结肠弯曲杆菌分离株中检测到对环丙沙星（氟喹诺酮类）的耐药性。全基因组测序揭示了多种序列类型（STs）。其中包括32种先前报道的空肠弯曲杆菌STs和13种结肠弯曲杆菌STs，以及每种细菌分别有4种和7种先前未描述的STs。含有耐氟喹诺酮分离株的STs为空肠弯曲杆菌的ST2083、ST10130、ST2895、ST7323、ST2398和ST1078，以及结肠弯曲杆菌的ST860和ST894。尽管氟喹诺酮类药物在澳大利亚的动物生产中不使用，但空肠弯曲杆菌分离株中的耐药性很高（24.4%）。这一发现强调需要加强监测并在食物链中定期采样，以了解分离株的来源并降低抗菌药物耐药性风险，从而保护公众健康。
