Shan Chaofan, Dong Kui, Wen Dongyu, Cui Zifan, Cao Jun
School of Life Sciences, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
School of Life Sciences, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
Int J Biol Macromol. 2025 May;308(Pt 2):142597. doi: 10.1016/j.ijbiomac.2025.142597. Epub 2025 Mar 27.
N6-methyladenosine (mA) represents the most prevalent internal modification observed in eukaryotic mRNAs. As a pivotal regulator of gene expression, mA exerts influence over a number of processes, including splicing, transport, translation, degradation, and the stability of mRNAs. It thus plays a crucial role in plant development and resistance to biotic and abiotic stressors. The writers, erasers, and readers of mA, which deposit, eliminate and decode this modification, are also of critical importance and have been identified and characterized in multiple plant species. The advent of next-generation sequencing (NGS) and mA detection technologies has precipitated a surge in research on mA in recent years. Extensive research has elucidated the specific roles of mA in plants and its underlying molecular mechanisms, indicating significant potential for crop improvement. This review presents a comprehensive overview of recent studies on mA and its regulatory proteins in plant development and stress tolerance. It highlights the potential applications of this modification and its writers, erasers, and readers for plant improvement, with a particular focus on leaf development, floral transition, trichome morphogenesis, fruit ripening, and resilience to pests, diseases and abiotic stresses.
N6-甲基腺嘌呤（m6A）是真核生物mRNA中最常见的内部修饰。作为基因表达的关键调节因子，m6A对许多过程产生影响，包括剪接、运输、翻译、降解以及mRNA的稳定性。因此，它在植物发育以及对生物和非生物胁迫的抗性中起着至关重要的作用。负责沉积、消除和解码这种修饰的m6A的写入器、擦除器和读取器也至关重要，并且已在多种植物物种中得到鉴定和表征。近年来，下一代测序（NGS）和m6A检测技术的出现促使了对m6A的研究激增。广泛的研究阐明了m6A在植物中的具体作用及其潜在的分子机制，显示出在作物改良方面的巨大潜力。本综述全面概述了近年来关于m6A及其调节蛋白在植物发育和胁迫耐受性方面的研究。它强调了这种修饰及其写入器、擦除器和读取器在植物改良中的潜在应用，特别关注叶片发育、花期转变、毛状体形态发生、果实成熟以及对病虫害和非生物胁迫的抗性。
