Yang Yanlan, Xiang Yang, Xu Pu, Zhang Wenbo, Wang Yawen, Feng Longbao, She Rong
Department of Oral Implantation, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou 570208, China.
Department of Hepatobiliary Surgery, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou 570208, China.
Colloids Surf B Biointerfaces. 2025 Aug;252:114651. doi: 10.1016/j.colsurfb.2025.114651. Epub 2025 Mar 27.
Bone defects, caused by trauma, osteomyelitis, or osteoporosis, represent a significant global health challenge in orthopedics. However, current bone repair strategies often neglect the critical role of the immune microenvironment, which can impede effective bone regeneration. To address this gap, we developed a 3D-printed triple crosslinked hydrogel scaffold incorporating slow-release glycopyrrolate (GA) and epigallocatechin gallate (EGCG), that it could promote bone regeneration by modulating the immune response. We evaluated their immunomodulatory and bone-regenerative effects through in vitro cellular experiments and rat cranial defect models. Results demonstrated that these scaffolds effectively modulated the immune microenvironment, reducing inflammation while promoting osteoblast differentiation and proliferation, thereby significantly enhancing new bone formation and density. In conclusion, our novel 3D-printed hydrogel scaffold offers a promising approach to bone defect repair through its unique combination of mechanical strength, immunomodulation, and osteogenesis. This study provides valuable insights into leveraging immunomodulatory agents for enhanced bone regeneration, highlighting potential clinical applications.
由创伤、骨髓炎或骨质疏松症引起的骨缺损是骨科领域一项重大的全球健康挑战。然而，当前的骨修复策略往往忽视了免疫微环境的关键作用，而这可能会阻碍有效的骨再生。为了弥补这一差距，我们开发了一种3D打印的三交联水凝胶支架，其包含缓释格隆溴铵（GA）和表没食子儿茶素没食子酸酯（EGCG），它可以通过调节免疫反应来促进骨再生。我们通过体外细胞实验和大鼠颅骨缺损模型评估了它们的免疫调节和骨再生作用。结果表明，这些支架有效地调节了免疫微环境，减少了炎症，同时促进了成骨细胞的分化和增殖，从而显著增强了新骨形成和骨密度。总之，我们新型的3D打印水凝胶支架通过其独特的机械强度、免疫调节和成骨作用的组合，为骨缺损修复提供了一种有前景的方法。这项研究为利用免疫调节药物增强骨再生提供了有价值的见解，突出了潜在的临床应用。
