Alkana R L, Finn D A, Galleisky G G, Bejanian M, Boone D C, Jones B, Syapin P J
Alcohol. 1985 Mar-Apr;2(2):281-5. doi: 10.1016/0741-8329(85)90060-6.
Findings in our laboratory indicate that body temperature alters ethanol potency as measured by sleep-time, wake-up brain ethanol concentration and mortality in male C57BL/6J mice. The current studies tested the generality of these results. Experiment one tested age-matched, drug-naive, male C57BL/6S and BALB/cS mice. Experiment two tested age-matched, drug-naive, male and female C57BL/6J mice. Each mouse was injected IP with 3.6 g/kg ethanol (20% w/v). After losing its righting reflex, the mouse was placed into a v-shaped sleep tray within a chamber kept at a designated temperature from 12 to 36 degrees C. Upon awakening, rectal temperature was measured and blood and brain samples were taken for gas chromatographic analysis. As in previous studies, ambient temperature modulated the body temperatures and sleep-times of intoxicated animals. More important, wake-up rectal temperatures were positively correlated with sleep-times and negatively correlated with wake-up ethanol concentrations in all animals tested. These results support the hypothesis that brain sensitivity to ethanol depression varies with body temperature in accordance with membrane perturbation theories of anesthesia.
我们实验室的研究结果表明，在雄性C57BL/6J小鼠中，体温会改变乙醇效力，这一效力通过睡眠时间、苏醒时大脑乙醇浓度和死亡率来衡量。当前的研究对这些结果的普遍性进行了测试。实验一测试了年龄匹配、未接触过药物的雄性C57BL/6S和BALB/cS小鼠。实验二测试了年龄匹配、未接触过药物的雄性和雌性C57BL/6J小鼠。每只小鼠腹腔注射3.6 g/kg乙醇（20% w/v）。在失去翻正反射后，将小鼠放入一个V形睡眠托盘中，该托盘置于一个温度保持在12至36摄氏度的指定温度的舱室内。苏醒后，测量直肠温度，并采集血液和大脑样本进行气相色谱分析。与之前的研究一样，环境温度调节了中毒动物的体温和睡眠时间。更重要的是，在所有测试的动物中，苏醒时的直肠温度与睡眠时间呈正相关，与苏醒时的乙醇浓度呈负相关。这些结果支持了这样一种假设，即根据麻醉的膜扰动理论，大脑对乙醇抑制的敏感性随体温而变化。
