Öndeş Baha, Aktaş Uygun Deniz
Aydın Adnan Menderes University, Faculty of Science, Department of Chemistry, Aydın, Türkiye.
Aydın Adnan Menderes University, Faculty of Science, Department of Chemistry, Aydın, Türkiye.
Anal Biochem. 2025 Jul;702:115857. doi: 10.1016/j.ab.2025.115857. Epub 2025 Mar 28.
In this study, a label-free nanowire-based impedimetric immunosensor was developed for the purpose of determining cancer biomarkers mucin 1 (MUC1). Nanowires were selected for sensor modification due to their high catalytic properties and high enzyme loading capacity. The synthesis, characterization, and application of Pt/Au/PPy-COOH nanowires to modify SPE electrodes were conducted. The nanowire-based immunosensors developed as a result of this research demonstrated a broad linear working range for MUC1 (20-3000 fg/mL), a low LOD value (0.244 fg/mL), and a low LOQ value (0.815 fg/mL). The nanowire-based immunosensor exhibited several notable characteristics. Firstly, it demonstrated excellent reproducibility, selectivity, and long-term stability. Furthermore, it demonstrated notable regenerative capabilities. It is noteworthy that the sensor exhibited the capability to detect MUC1 in commercial human serum samples, even in the presence of interfering agents. The affordability, simplicity, and expeditious analysis of nanowire-based immunosensors render them more appealing than alternative commercial kits. Consequently, these sensors hold considerable promise for clinical applications.
在本研究中，为了测定癌症生物标志物粘蛋白1（MUC1），开发了一种基于无标记纳米线的阻抗免疫传感器。由于纳米线具有高催化性能和高酶负载能力，因此选择其对传感器进行修饰。开展了Pt/Au/PPy-COOH纳米线修饰SPE电极的合成、表征及应用研究。本研究开发的基于纳米线的免疫传感器对MUC1显示出较宽的线性工作范围（20 - 3000 fg/mL）、较低的检测限（0.244 fg/mL）和较低的定量限（0.815 fg/mL）。基于纳米线的免疫传感器具有几个显著特点。首先，它表现出优异的重现性、选择性和长期稳定性。此外，它还表现出显著的再生能力。值得注意的是，即使存在干扰剂，该传感器仍具有在商业人血清样本中检测MUC1的能力。基于纳米线的免疫传感器价格实惠、操作简单且分析迅速，使其比其他商业试剂盒更具吸引力。因此，这些传感器在临床应用中具有很大的前景。
