Beck-Speier I, Hinze H, Holzer H
Biochim Biophys Acta. 1985 Jul 26;841(1):81-9. doi: 10.1016/0304-4165(85)90276-4.
Mammalian tissues show significant differences in the activity of sulfite oxidase (EC 1.8.3.1) which detoxifies sulfite by oxidation to sulfate. Lung tissue and phagocytic cells such as alveolar macrophages, peritoneal macrophages, Kupffer cells and granulocytes show very low activities of sulfite oxidase. Liver tissue and hepatocytes, however, exhibit high activities of sulfite oxidase. Lung tissue and macrophages show an almost 100% decrease of the intracellular ATP levels when incubated with 1 mM sulfite at pH 6 for 30 min. In addition, the O2 consumption of lung tissue is inhibited by 1 mM sulfite at pH 6 by more than 80%. This sulfite-induced decrease of the ATP level and of the O2 consumption of lung tissue is enhanced between pH 6.0 and pH 7.4 with decreasing pH value of the incubation medium. In contrast, the ATP levels in liver tissue and hepatocytes are not affected by 1 mM sulfite at pH 6. The O2 consumption of liver tissue and hepatocytes is significantly increased by sulfite due to the high activities of sulfite oxidase. Therefore, the activity of the 'sulfite-detoxifying enzyme' sulfite oxidase and the sensitivity of the energy metabolism to sulfite show a reciprocal relationship in the tissues and cells studied.
哺乳动物组织在亚硫酸盐氧化酶（EC 1.8.3.1）的活性上表现出显著差异，该酶通过将亚硫酸盐氧化为硫酸盐来解毒。肺组织和吞噬细胞，如肺泡巨噬细胞、腹膜巨噬细胞、库普弗细胞和粒细胞，亚硫酸盐氧化酶的活性非常低。然而，肝组织和肝细胞表现出较高的亚硫酸盐氧化酶活性。当在pH 6条件下与1 mM亚硫酸盐孵育30分钟时，肺组织和巨噬细胞的细胞内ATP水平几乎降低100%。此外，在pH 6条件下，1 mM亚硫酸盐可使肺组织的耗氧量降低80%以上。随着孵育介质pH值从6.0降至7.4，亚硫酸盐诱导的肺组织ATP水平降低和耗氧量降低会增强。相比之下，在pH 6条件下，1 mM亚硫酸盐不会影响肝组织和肝细胞中的ATP水平。由于亚硫酸盐氧化酶的高活性，亚硫酸盐可显著增加肝组织和肝细胞的耗氧量。因此，在所研究的组织和细胞中，“亚硫酸盐解毒酶”亚硫酸盐氧化酶的活性与能量代谢对亚硫酸盐的敏感性呈反比关系。
