Arora Kanika, Celejewski Konrad, Narang Dominic
University of Waterloo, Waterloo, ON, Canada.
HDX-MS Department, Rapid Novor Inc, Kitchener, ON, Canada.
Methods Mol Biol. 2025;2905:181-192. doi: 10.1007/978-1-0716-4418-8_12.
Hydrogen-deuterium exchange mass spectrometry (HDX-MS) is an invaluable analytical tool in structural biology and biochemistry, significantly enhancing our understanding of protein dynamics and interactions. This technique utilizes heavy water (DO) to label the protein molecules and employs a mass spectrometer to measure the extent of deuterium incorporation in various protein regions. The extent of deuterium incorporation provides valuable insights into protein structure, conformational dynamics, and interactions, making it a powerful tool for identifying the binding sites, epitopes, and comparative analyses. HDX-MS is a powerful research tool and a catalyst for innovative drug discoveries and advancements in various scientific fields.
氢-氘交换质谱法（HDX-MS）是结构生物学和生物化学中一种非常宝贵的分析工具，极大地增进了我们对蛋白质动力学和相互作用的理解。该技术利用重水（D₂O）标记蛋白质分子，并使用质谱仪测量不同蛋白质区域中氘的掺入程度。氘的掺入程度为蛋白质结构、构象动力学和相互作用提供了有价值的见解，使其成为识别结合位点、表位以及进行比较分析的强大工具。HDX-MS是一种强大的研究工具，也是各科学领域创新药物发现和进步的催化剂。
