农业害虫粘虫的高质量染色体水平基因组组装

A high-quality chromosome-level genome assembly for the agricultural pest Mythimna separata.

作者信息

Zhang Lei, Yesaya Alexander, Li Zaiyuan, Liang Xinyue, Xiao Yutao

机构信息

Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, 518120, Shenzhen, China.

出版信息

Sci Data. 2025 Mar 31;12(1):540. doi: 10.1038/s41597-025-04855-7.

DOI:10.1038/s41597-025-04855-7

PMID:40164652

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11958816/

Abstract

The oriental armyworm, Mythimna separata, poses a persistent challenge to agricultural pest management due to its strong migratory abilities and polyphagous feeding behavior. In this study, we present a chromosome-level genome assembly using Illumina, PacBio HiFi, and Hi-C sequencing technologies. The final assembly spans 714.5 Mb with a scaffold N50 of 22.7 Mb and a GC content of 38.8%. A total of 32 chromosomes were successfully anchored, including the Z and W sex chromosomes. BUSCO analysis indicated a genome completeness of 98.6%, and 19,879 protein-coding genes were predicted. The W chromosome, measuring 30.55 Mb with a repeat content of 68.34%, harbors 824 protein-coding genes. Furthermore, a PCR-based method confirmed W-linked sequences for female-specific sex detection via the ZW system. This enhanced genome assembly provides a valuable resource for evolutionary research on M. separata and facilitates the development of sex-regulated pest control strategies.

摘要

东方黏虫（Mythimna separata）因其强大的迁徙能力和多食性取食行为，对农业害虫管理构成了持续挑战。在本研究中，我们使用Illumina、PacBio HiFi和Hi-C测序技术进行了染色体水平的基因组组装。最终组装结果跨度为714.5 Mb，支架N50为22.7 Mb，GC含量为38.8%。共成功锚定了32条染色体，包括Z和W性染色体。BUSCO分析表明基因组完整性为98.6%，预测有19,879个蛋白质编码基因。W染色体大小为30.55 Mb，重复序列含量为68.34%，含有824个蛋白质编码基因。此外，一种基于PCR的方法通过ZW系统确认了用于雌性特异性性别检测的W连锁序列。这种改进的基因组组装为东方黏虫的进化研究提供了宝贵资源，并促进了性别调控害虫控制策略的发展。