Zhang Yi, Guo Zhipeng, Sun Jie, Bi Yingwen, Cai Rongrong, Zhang Rui, Ren Hui, Qian Jiang, Meng Fengxi
Department of Ophthalmology, Eye & ENT Hospital of Fudan University, Shanghai, China.
Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China.
BMC Cancer. 2025 Mar 31;25(1):581. doi: 10.1186/s12885-025-13438-z.
To quantitatively investigate the pathological subtypes of lacrimal gland adenoid cystic carcinoma (LGACCs), the tumor immune microenvironment in each pathological subtype, and the relation to survival.
In this retrospective study, the tumor subtype was determined by H&E staining. Multiplex immunochemistry was performed to define specific immune cells. The tumor immune microenvironment (TIME) was sketched by sequential image scanning and reconstructed by a cytometry platform.
Eighteen patients with adequate paraffin blocks diagnosed with LGACC from 2012 to 2021 were included in this study. Thirteen patients out of the eighteen patients (72.2%)showed a mixture of different pathological subtypes. Each pathological subtype took different percentages on different tumors. The cribriform was the most common subtype, taking an overall percentage of 39%. The rest of the pathological subtypes were tubular (19%), basaloid (17%), cribriform and tubular mixture (C + T) 14%. The sclerosing and comedocarcinomic subtypes were the least seen in LGACC, taking a percentage of 11% altogether. Patients with cribriform dominant component had better overall survival than the non-cribriform dominant patients. Patients with basaloid dominant component had worse clinical outcomes than the non-basaloid dominant ones. The TIME showed high immunogenicity in the tumor margin but declined in the tumor areas. Pathological subtypes rather than individual differences determined the TIME phenotype. The cribriform subtype possessed more immune cell infiltration than other pathological subtypes.
LGACC is composed of multiple pathological subtypes. Each pathological subtype takes different percentages on different tumors, which is related to the prognosis. TIME pattern in LGACC varies among different pathological subtypes, which could indicate novel strategies in immunotherapy.
定量研究泪腺腺样囊性癌（LGACC）的病理亚型、各病理亚型中的肿瘤免疫微环境及其与生存的关系。
在这项回顾性研究中，通过苏木精-伊红（H&E）染色确定肿瘤亚型。进行多重免疫化学以鉴定特定免疫细胞。通过序列图像扫描勾勒肿瘤免疫微环境（TIME），并通过细胞计数平台进行重建。
本研究纳入了2012年至2021年诊断为LGACC且石蜡块充足的18例患者。18例患者中有13例（72.2%）表现出不同病理亚型的混合。各病理亚型在不同肿瘤中所占比例不同。筛状型是最常见的亚型，总体占比39%。其余病理亚型为管状型（19%）、基底样型（17%）、筛状和管状混合型（C+T）14%。硬化型和粉刺癌型在LGACC中最少见，共占11%。筛状为主成分的患者总生存期优于非筛状为主的患者。基底样为主成分的患者临床结局比非基底样为主的患者差。TIME在肿瘤边缘显示高免疫原性，但在肿瘤区域下降。病理亚型而非个体差异决定了TIME表型。筛状亚型比其他病理亚型具有更多的免疫细胞浸润。
LGACC由多种病理亚型组成。各病理亚型在不同肿瘤中所占比例不同，这与预后相关。LGACC中TIME模式在不同病理亚型间存在差异，这可能为免疫治疗指明新策略。
