Anticancer, Antioxidant and Antimicrobial Activity of . Leaf Extract.

OBJECTIVE

This study was conducted to determine and compare the antioxidant, cytotoxic, and antimicrobial effects of spindle leaves of L. () (oleaster) leaves.

METHODS

Total phenolic content was measured using the Folin-Ciocalteu method, phenolic compound analysis by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) and antimicrobial effect by the minimum inhibition concentration (MIC) method. The free radical scavenging activity was determined by t (2,2-Diphenyl-1-picrylhydrazyl) DPPH method, the free radical scavenging activity was determined by the ABTS method, and cytotoxicity assays were performed by the MTT method in human retinal epithelium cells (RPE-1), human osteosarcoma cells (U2OS), and prostate cancer cells (DU-145) cell lines.

RESULTS

High amounts of gallic acid, protocatechuic acid, and o-coumaric acid were identified as phenolic compounds. was found to have a good antioxidant capacity and high free radical scavenging capacity. In this study, for the first time, leaf extract was used to investigate cytotoxic effects on human retinal epithelium (RPE-1), human osteosarcoma cells (U2OS), and prostate cancer (DU-145) cells and antimicrobial effects on American Type Culture Collection (ATTC) 7644, Staphylococcus aureus ATCC 29213, ATCC 25922, ATCC 11774, and ATCC 10231 microorganisms. The highest cytotoxic effect was observed in the DU-145 cell line, and the highest antimicrobial effect was observed in ATTC 7644 and ATCC 10231. The leaf extract of the plant contains some important phenolic compounds and has high free radical scavenging capacity, a good anticancer effect, and effective antimicrobial activity on yeast species such as .

CONCLUSION

Our study will contribute greatly to the search for anticancer and antimicrobial agents, especially from a pharmacological perspective, by examining biological activity using three different methods.