15N标记的N-亚硝基脯氨酸在离体大鼠肝细胞及亚细胞组分中的代谢研究。

Metabolic studies of 15N-labeled N-nitrosoproline in isolated rat hepatocytes and subcellular fractions.

作者信息

Koepke S R, Tondeur Y, Farrelly J G, Stewart M L, Michejda C J, Kroeger-Koepke M B

出版信息

Cancer Lett. 1985 Jul;27(3):277-83. doi: 10.1016/0304-3835(85)90185-5.

Abstract

The metabolism of the non-carcinogenic N-nitrosoproline (NPRO) was investigated in vitro using both S9 preparations and isolated hepatocytes from F344 rats. The studies were performed using 15N-labeled nitrosamine and the reaction mixtures were examined mass spectrometrically for the presence of 15N2 or other 15N-labeled gaseous products. In addition, the metabolism of NPRO was monitored by capillary gas chromatography. The results indicated no 15N2 production from either the hepatocyte or S9 preparations, as well as no detectable loss of substrate from the reaction mixtures. Mass spectrometric analysis failed to reveal any metabolites of NPRO. The results suggest that NPRO may be refractory to the normal nitrosamine activating enzymes, confirming its suitability for use in human epidemiological studies of endogenous nitrosation.

摘要

使用来自F344大鼠的S9制剂和分离的肝细胞，在体外研究了非致癌性N-亚硝基脯氨酸（NPRO）的代谢。研究使用15N标记的亚硝胺进行，反应混合物通过质谱法检测15N2或其他15N标记的气态产物的存在。此外，通过毛细管气相色谱法监测NPRO的代谢。结果表明，肝细胞或S9制剂均未产生15N2，反应混合物中也未检测到底物损失。质谱分析未能揭示NPRO的任何代谢产物。结果表明，NPRO可能对正常的亚硝胺活化酶具有抗性，证实了其适用于内源性亚硝化的人类流行病学研究。