Demecsová Loriana, Liptáková Ľubica, Valentovičová Katarína, Zelinová Veronika, Tamás Ladislav
Institute of Botany, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Dúbravská Cesta 9, 84523, Bratislava, Slovak Republic.
Protoplasma. 2025 Apr 1. doi: 10.1007/s00709-025-02058-w.
In this study, using a pharmaceutical approach, we analyzed the NO accumulation and emission from the root tips of barley seedlings and the possible mechanisms of NO catabolism. Application of flavohemeprotein inhibitors, such as azide, cyanide, diphenyleneiodonium and dicumarol, an inhibitor of the plasma membrane electron transport chain, increased the NO level in root tissue and stimulated the NO emission from root tip cells. It can be concluded that barley root tips generate and, at the same time, consume a considerable amount of NO, probably by the plasma membrane flavohemeproteins. This high NO-consuming activity of barley root tips efficiently degraded even the externally applied high concentrations of NO without marked root growth inhibition. These results suggest that the root tip cells NO consumption activity plays an important role in the regulation of NO level in barley root tips.
在本研究中，我们采用药物学方法分析了大麦幼苗根尖中一氧化氮（NO）的积累与释放以及NO分解代谢的可能机制。应用黄素血红蛋白抑制剂，如叠氮化物、氰化物、二苯基碘鎓和双香豆素（一种质膜电子传递链抑制剂），可提高根组织中的NO水平，并刺激根尖细胞释放NO。可以得出结论，大麦根尖可能通过质膜黄素血红蛋白产生并同时消耗大量的NO。大麦根尖这种高NO消耗活性甚至能有效降解外部施加的高浓度NO，且对根系生长无明显抑制作用。这些结果表明，根尖细胞的NO消耗活性在调节大麦根尖NO水平中起着重要作用。
