硝酸还原酶调节植物一氧化氮稳态。

Nitrate Reductase Regulates Plant Nitric Oxide Homeostasis.

机构信息

Department of Biochemistry and Molecular Biology, University of Cordoba, Campus de Rabanales, School of Sciences, Campus de Excelencia Internacional (CeiA3), Edifico Severo Ochoa, Cordoba, Spain.

Department of Biochemistry and Molecular Biology, University of Cordoba, Campus de Rabanales, School of Sciences, Campus de Excelencia Internacional (CeiA3), Edifico Severo Ochoa, Cordoba, Spain; Present address: Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA.

出版信息

Trends Plant Sci. 2017 Feb;22(2):163-174. doi: 10.1016/j.tplants.2016.12.001. Epub 2017 Jan 5.

DOI:10.1016/j.tplants.2016.12.001

PMID:28065651

Abstract

Nitrate reductase (NR) is a key enzyme for nitrogen acquisition by plants, algae, yeasts, and fungi. Nitrate, its main substrate, is required for signaling and is widely distributed in diverse tissues in plants. In addition, NR has been proposed as an important enzymatic source of nitric oxide (NO). Recently, NR has been shown to play a role in NO homeostasis by supplying electrons from NAD(P)H through its diaphorase/dehydrogenase domain both to a truncated hemoglobin THB1, which scavenges NO by its dioxygenase activity, and to the molybdoenzyme NO-forming nitrite reductase (NOFNiR) that is responsible for NO synthesis from nitrite. We review how NR may play a central role in plant biology by controlling the amounts of NO, a key signaling molecule in plant cells.

摘要

硝酸还原酶 (NR) 是植物、藻类、酵母和真菌获取氮的关键酶。其主要底物硝酸盐是信号传导所必需的，并且广泛分布在植物的各种组织中。此外，NR 已被提议作为一氧化氮 (NO) 的重要酶源性物质。最近，NR 通过其黄递酶/脱氢酶结构域将 NAD(P)H 中的电子提供给截断的血红蛋白 THB1，从而在 NO 稳态中发挥作用，THB1 通过其加双氧酶活性清除 NO，以及钼酶形成亚硝酸盐的一氧化氮还原酶 (NOFNiR)，负责从亚硝酸盐合成 NO。我们回顾了 NR 如何通过控制细胞内关键信号分子 NO 的量来在植物生物学中发挥核心作用。