重组质粒热休克蛋白47小干扰核糖核酸对兔眼滤过手术后滤过通道瘢痕形成的影响。

Effect of recombinant plasmid heat shock protein 47 small interfering ribonucleic acid on the scarring of the filtering channel after filtration surgery in rabbit eyes.

作者信息

Gan Chunfang, Yao Shasha, Pan Rugang

机构信息

Department of Ophthalmology, Ya'an People's Hospital, No. 9 Ankang Road, Yucheng District, Ya'an City, 625000, Sichuan Province, China.

出版信息

Int Ophthalmol. 2025 Apr 1;45(1):137. doi: 10.1007/s10792-025-03511-8.

DOI:10.1007/s10792-025-03511-8

PMID:40167871

Abstract

OBJECTIVE

This study aimed to investigate the effects of recombinant plasmid heat shock protein 47 small interfering ribonucleic acid (HSP47-siRNA) on fibroblast proliferation and collagenous fibre synthesis in the filtering channel after trabeculectomy in rabbit.

METHODS

The recombinant plasmid HSP47-siRNA was constructed successfully to extract sufficient recombinant plasmids. Furthermore, an animal model of anti-glaucoma surgery in rabbit eyes was created to establish the HSP47-siRNA group, empty vector group, mitomycin C (MMC) group and normal saline group. This study further observed the postoperative eye condition, measured the intraocular pressure and carried out histological and immunohistochemical staining analysis.

RESULTS

At different time points after surgery, there were no significant differences in the comparison of filtering bleb morphology, conjunctival congestion, corneal oedema, inflammation and depth of anterior chamber or lens opacity among the HSP47-siRNA, empty vector, MMC and normal saline groups. Within 7 days after surgery, filtering blebs in all groups were bulged and diffused, with no statistically significant difference (p > 0.05). On day 15 and day 30 after surgery, the upper filtering bleb gradually decreased in the empty vector group and the normal saline group compared with the HSP47-siRNA group (p < 0.01). On day 30 after surgery, flat and pale scar tissues were observed in the surgical area of the empty vector and normal saline groups, whereas functional blebs were still observed in the HSP47-siRNA and MMC groups. Within 7 days after surgery, the intraocular pressure significantly decreased in each group, with a statistically significant difference compared with that before surgery (p < 0.01).

CONCLUSION

HSP47-siRNA exhibits application potential concerning its effects on anti-scarring after glaucoma filtration surgery in rabbit.

摘要

目的

本研究旨在探讨重组质粒热休克蛋白47小干扰核糖核酸（HSP47-siRNA）对兔小梁切除术后滤过通道成纤维细胞增殖及胶原纤维合成的影响。

方法

成功构建重组质粒HSP47-siRNA以提取足够的重组质粒。此外，建立兔眼抗青光眼手术动物模型，设立HSP47-siRNA组、空载体组、丝裂霉素C（MMC）组和生理盐水组。本研究进一步观察术后眼部情况，测量眼压，并进行组织学和免疫组化染色分析。

结果

术后不同时间点，HSP47-siRNA组、空载体组、MMC组和生理盐水组在滤过泡形态、结膜充血、角膜水肿、炎症及前房深度或晶状体混浊程度的比较上无显著差异。术后7天内，各组滤过泡均隆起且弥散，差异无统计学意义（p＞0.05）。术后第15天和第30天，与HSP47-siRNA组相比，空载体组和生理盐水组上方滤过泡逐渐减小（p＜0.01）。术后第30天，空载体组和生理盐水组手术区域可见扁平、苍白的瘢痕组织，而HSP47-siRNA组和MMC组仍可见功能性滤过泡。术后7天内，各组眼压均显著降低，与术前相比差异有统计学意义（p＜0.01）。