Ibrahim Samar Sayed, El-Kholy Magdy Youssef, Shalaby Shehata El-Sayed Mohamed
Pests and Plant Protection Department, National Research Centre, 33 El-Buhouth Street, Dokki, 12622, Giza, Egypt.
Sci Rep. 2025 Apr 1;15(1):11138. doi: 10.1038/s41598-025-93779-8.
Essential oils derived from plants have demonstrated their efficacy in controlling insects; however, their poor physical characteristics restrict their widespread application. In this study, nano-encapsulation employing emulsification followed by ultrasonication technique has improved the insecticidal efficacy of lemongrass oil (LGO) against Spodoptera frugiperda. Beta-cyclodextrin (β-CD) and gum Arabic (GA) were used as coating materials to protect the vital components of LGO. Different concentrations of LGO pure and nano-capsule formulations have been used to examine the biological development of S. frugiperda 1st larval instar using two sex life table. D-Limonene, E-Citral, and Z-Citral were identified as the main compounds in LGO by GC-MS analysis. The oil concentration affected the physical properties of nano-capsules. The LGO nano-capsules had particle sizes ranging from 279.63 to 471.93 nm and an encapsulation efficiency between 51.94 and 83.59%. Following 96 h, the LGO:β-CD/GA treatment of S. frugiperda had a lower LC value (1.52%) than the LGO treatment (2.48%), indicating that the nano-capsules were more toxic. Nano-capsules had a significant adverse effect on the life table parameters of S. frugiperda. The results of this study indicate that encapsulating LGO with β-CD/GA inclusion enhanced the efficacy of lemongrass oil against S. frugiperda.
从植物中提取的精油已证明其在控制昆虫方面的功效；然而，其较差的物理特性限制了它们的广泛应用。在本研究中，采用乳化后超声处理技术的纳米包封提高了柠檬草油（LGO）对草地贪夜蛾的杀虫效果。β-环糊精（β-CD）和阿拉伯胶（GA）被用作包衣材料以保护LGO的重要成分。使用两种性生命表，采用不同浓度的LGO纯品和纳米胶囊制剂来研究草地贪夜蛾1龄幼虫的生物学发育。通过气相色谱-质谱联用（GC-MS）分析确定D-柠檬烯、E-柠檬醛和Z-柠檬醛是LGO中的主要化合物。油浓度影响纳米胶囊的物理性质。LGO纳米胶囊的粒径范围为279.63至471.93nm，包封效率在51.94%至83.59%之间。96小时后，LGO:β-CD/GA处理草地贪夜蛾的LC值（1.52%）低于LGO处理（2.48%），表明纳米胶囊毒性更大。纳米胶囊对草地贪夜蛾的生命表参数有显著不利影响。本研究结果表明，用β-CD/GA包合LGO可提高柠檬草油对草地贪夜蛾的防治效果。
