Madalena Isabela Ribeiro, Küchler Erika Calvano, Reis Caio Luiz Bitencourt, Matsumoto Mirian Aiko Nakane, Stuani Maria Bernadete Sasso, Vilalba Paniagua Machado do Nascimento Thaís, Kirschneck Christian, Baratto-Filho Flares, Menezes-Oliveira Maria Angélica Hueb de, Lepri César Penazzo
Universidade de Uberaba - Uniube, School of Dentistry, Department of Biomaterials, Uberaba, MG, Brazil.
University Hospital Bonn, School of Medicine, Department of Orthodontics, Bonn, Germany.
Braz Oral Res. 2025 Mar 31;39:e033. doi: 10.1590/1807-3107bor-2025.vol39.033. eCollection 2025.
The aim was to evaluate the association between dental development (dental maturity) and genetic polymorphisms in PTH and genes involved in vitamin D synthesis in a cohort of Brazilian children. This retrospective cross-sectional study was performed on children receiving orthodontic treatment. Patients who had already undergone orthodontic treatment previously, those with syndromes, congenital anomalies, craniofacial deformities, and those with a previous history of dental trauma and bilateral agenesis/missing tooth/teeth were excluded. Panoramic radiographs were used for dental age evaluation according to the method proposed by Demirjian, Goldstein, and Tanner (1976). A delta [dental age minus chronological age (DA-CA)] was calculated to determine whether the patient's dental age was normal, delayed (negative values), or advanced (positive values). DNA isolated from buccal cells was used for genotyping genetic polymorphisms in PTH (rs694, rs307247, and rs6256), VDR (rs7975232), CYP27B1 (rs464653), and CYP24A1 (rs927650). A statistical analysis was performed and p<0.05 indicated statistical difference. A total of 79 orthodontic patients were included (44 (55.70%) girls and 35 (44.30%) boys). Demirjian, Goldstein, and Tanner's method (1976) overestimated the age of patients by 0.75 years. None of the genetic polymorphisms were associated with dental age (p>0.05). In conclusion, there is no association between genetic polymorphisms in PTH and genes involved in vitamin D synthesis and dental maturity.
本研究旨在评估巴西儿童队列中牙齿发育（牙齿成熟度）与甲状旁腺激素（PTH）基因多态性以及参与维生素D合成的基因之间的关联。这项回顾性横断面研究是针对接受正畸治疗的儿童进行的。已接受过正畸治疗的患者、患有综合征、先天性异常、颅面畸形的患者，以及有牙齿外伤和双侧牙胚缺失/缺牙病史的患者被排除在外。根据Demirjian、Goldstein和Tanner（1976年）提出的方法，使用全景X线片评估牙齿年龄。计算差值[牙齿年龄减去实际年龄（DA-CA）]以确定患者的牙齿年龄是否正常、延迟（负值）或提前（正值）。从颊细胞中分离的DNA用于对PTH（rs694、rs307247和rs6256）、维生素D受体（VDR，rs7975232）、细胞色素P450 27B1（CYP27B1，rs464653）和细胞色素P450 24A1（CYP24A1，rs927650）的基因多态性进行基因分型。进行了统计分析，p<0.05表示有统计学差异。总共纳入了79名正畸患者（44名（55.70%）女孩和35名（44.30%）男孩）。Demirjian、Goldstein和Tanner的方法（1976年）将患者年龄高估了0.75岁。没有一种基因多态性与牙齿年龄相关（p>0.05）。总之，PTH基因多态性以及参与维生素D合成的基因与牙齿成熟度之间不存在关联。
