Department of Orthodontics, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.
Department of Orthodontics, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany; School of Dentistry, Tuiuti University from Paraná, Curitiba, Paraná, Brazil.
Ann Anat. 2022 Oct;244:151972. doi: 10.1016/j.aanat.2022.151972. Epub 2022 Jun 20.
The present study aimed to evaluate if functional genetic polymorphisms in vitamin-D-related genes are associated with third molar agenesis and third molar microdontia in German orthodontic patients. Pre-orthodontic and follow-up treatment records were evaluated for phenotype definition. Saliva samples were collected for DNA extraction. Eight potential functional genetic polymorphisms in VDR [rs731236 (TaqI), rs7975232 (ApaI), rs2228570 (FokI), and rs1544410 (BsmI)], CYP27B1 (rs4646536), CYP24A1 (rs927650), GC (rs4588), and SEC23A (rs8018720) were evaluated using real-time PCR. Comparison among the groups were performed (third molar anomaly vs. control; third molar agenesis vs. control; and third molar microdontia vs. control) with an alpha of 5%. A total of 164 patients were analyzed. Forty-nine (29.9%) patients had at least one third molar anomaly. In the haplotype analysis, genetic polymorphisms in VDR and CYP27B1 were associated with third molar anomalies (p < 0.05). The G allele in rs8018720 (SEC23A) was more frequent in microdontia cases. In the genotype distribution analysis, rs8018720 in SEC23A was associated with third molar microdontia in the co-dominant (p = 0.034; Prevalence Ratio [PR]=5.91, 95% Confidence Interval [CI]= 1.14-30.66) and in the recessive (p = 0.038; PR=5.29; 95% CI= 1.09-25.65) models. In conclusion, vitamin D-related genes could be involved in third molar anomalies.
本研究旨在评估维生素 D 相关基因的功能遗传多态性是否与德国正畸患者的第三磨牙缺失和第三磨牙过小有关。为了定义表型，评估了正畸前和随访治疗记录。收集唾液样本进行 DNA 提取。使用实时 PCR 评估 VDR [rs731236（TaqI）、rs7975232（ApaI）、rs2228570（FokI）和 rs1544410（BsmI）]、CYP27B1（rs4646536）、CYP24A1（rs927650）、GC（rs4588）和 SEC23A（rs8018720）中的 8 个潜在功能遗传多态性。在α为 5%的情况下，对各组（第三磨牙异常与对照组；第三磨牙缺失与对照组；第三磨牙过小与对照组）进行了比较。共分析了 164 名患者。49 名（29.9%）患者至少有一颗第三磨牙异常。在单倍型分析中，VDR 和 CYP27B1 的遗传多态性与第三磨牙异常有关（p<0.05）。rs8018720（SEC23A）中的 G 等位基因在过小病例中更为常见。在基因型分布分析中，SEC23A 中的 rs8018720 在共显性（p=0.034；优势比[PR]=5.91，95%置信区间[CI]=1.14-30.66）和隐性（p=0.038；PR=5.29；95%CI=1.09-25.65）模型中与第三磨牙过小有关。综上所述，维生素 D 相关基因可能参与第三磨牙异常。
