Tomford W W, Duff G P, Mankin H J
Clin Orthop Relat Res. 1985 Jul-Aug(197):11-4.
Preservation of articular cartilage chondrocytes is currently being investigated in conjunction with storage and transplantation of osteochondral allografts. Studies on isolated chondrocytes have suggested that viability and function may be retained following freeze-preservation. Up to 90% of frozen isolated cells will survive, and these cells are capable of producing proteoglycans in culture. Results of freezing chondrocytes in a matrix have not been as successful, with viability ranging from 0% to 50%. Inconsistent results are thought to be due to the matrix and associated problems such as poor penetration of the cryopreservative, an unequal rate of cooling, and a high water content. Studies are in progress in an attempt to produce a satisfactory method of freeze-preservation that will promote normal functioning of chondrocytes after transplantation in an articular cartilage matrix.
目前正在结合骨软骨异体移植的储存和移植来研究关节软骨软骨细胞的保存。对分离软骨细胞的研究表明，冷冻保存后其活力和功能可能得以保留。高达90%的冷冻分离细胞能够存活，并且这些细胞在培养中能够产生蛋白聚糖。在基质中冷冻软骨细胞的结果并不那么成功，存活率在0%至50%之间。结果不一致被认为是由于基质以及相关问题，如冷冻保护剂渗透不佳、冷却速度不均和高含水量。目前正在进行研究，试图找到一种令人满意的冷冻保存方法，以促进软骨细胞在关节软骨基质中移植后正常发挥功能。
