从黎凡特蝰蛇（蛇）毒液中分离和鉴定神经生长因子。
Isolation and characterization of nerve growth factor from Vipera lebetina (snake) venom.
作者信息
Siigur E, Neuman T, Järve V, Tara A, Siigur J
出版信息
Comp Biochem Physiol B. 1985;81(1):211-5. doi: 10.1016/0305-0491(85)90185-3.
PMID:4017541
Abstract
Nerve growth factor from Vipera lebetina venom was purified by gel filtration and ion exchange chromatography steps. The NGF preparation obtained is a glycoprotein with weak arginine esterase activity. It hydrolyzes benzoylarginine ethyl ester (BAEE). Vipera lebetina NGF consists of multiple forms of protein with pI in the interval 9-10.5. All isoforms have identical molecular weights of 32,500.
摘要
通过凝胶过滤和离子交换色谱步骤对来自黎凡特蝰蛇毒液的神经生长因子进行了纯化。所获得的神经生长因子制剂是一种具有弱精氨酸酯酶活性的糖蛋白。它能水解苯甲酰精氨酸乙酯（BAEE）。黎凡特蝰蛇神经生长因子由多种形式的蛋白质组成，其等电点在9 - 10.5之间。所有同工型的分子量均为32,500。
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