从极北蝰（普通蝰蛇）毒液中分离和鉴定神经生长因子。

Isolation and characterization of nerve growth factor from Vipera berus berus (common viper) venom.

作者信息

Siigur J, Arumäe U, Neuman T, Samel M, Siigur E, Saarma M

出版信息

Comp Biochem Physiol B. 1986;83(3):621-5. doi: 10.1016/0305-0491(86)90306-8.

Abstract

Nerve growth factor from Vipera berus berus venom was purified by gel filtration on Sephadex G-100 (superfine), ion-exchange-chromatography on DEAE-Sephadex A-50 and chromatofocusing on PBE 118. The Vipera berus berus venom NGF consists of multiple molecular forms with pls in the interval 9.1-9.7. All isoforms have identical mol. wts approximately 35,000 +/- 3000 (in gel filtration) and 17,000 +/- 2000, 15,000 +/- 2000 (by SDS electrophoresis with beta-mercaptethanol). V. berus berus venom NGF reacted with monoclonal antibodies against Viper lebetina NGF and caused differentiation of pheochromocytoma PC12 cells.

摘要

通过在Sephadex G - 100（超细）上进行凝胶过滤、在DEAE - Sephadex A - 50上进行离子交换色谱以及在PBE 118上进行色谱聚焦，从极北蝰蛇毒中纯化出神经生长因子。极北蝰蛇毒神经生长因子由多种分子形式组成，其等电点在9.1 - 9.7范围内。所有同工型具有大致相同的分子量，在凝胶过滤中约为35,000 +/- 3000，在使用β - 巯基乙醇的SDS电泳中为17,000 +/- 2000、15,000 +/- 2000。极北蝰蛇毒神经生长因子与抗草原蝰蛇神经生长因子的单克隆抗体发生反应，并导致嗜铬细胞瘤PC12细胞分化。