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丁香假单胞菌亚群通过协调鞭毛和III型分泌的时空动态来合作，以促进对植物的感染。

Pseudomonas syringae subpopulations cooperate by coordinating flagellar and type III secretion spatiotemporal dynamics to facilitate plant infection.

作者信息

López-Pagán Nieves, Rufián José S, Luneau Julien, Sánchez-Romero María-Antonia, Aussel Laurent, van Vliet Simon, Ruiz-Albert Javier, Beuzón Carmen R

机构信息

Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Málaga, Spain.

Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic.

出版信息

Nat Microbiol. 2025 Apr;10(4):958-972. doi: 10.1038/s41564-025-01966-0. Epub 2025 Apr 2.

DOI:10.1038/s41564-025-01966-0
PMID:40175722
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11964935/
Abstract

Isogenic bacterial populations can display probabilistic cell-to-cell variation in response to challenges. This phenotypic heterogeneity can affect virulence in animals, but its impact on plant pathogens is unknown. Previously, we showed that expression of the type III secretion system (T3SS) of the plant pathogen Pseudomonas syringae displays phenotypic variation in planta. Here we use flow cytometry and microscopy to investigate single-cell flagellar expression in relation to T3SS expression, showing that both systems undergo phenotypic heterogeneity in vitro in apoplast-mimicking medium and within apoplastic microcolonies throughout colonization of Phaseolus vulgaris. Stochastic, spatial and time factors shape the dynamics of a phenotypically diverse pathogen population that displays division of labour during colonization: effectors produced by T3SS-expressing bacteria act as 'common goods' to suppress immunity, allowing motile flagella-expressing bacteria to increase and leave infected tissue before necrosis. These results showcase the mechanisms of bacterial specialization during plant colonization in an environmentally and agriculturally relevant system.

摘要

同基因细菌群体在应对挑战时可能会表现出细胞间的概率性变异。这种表型异质性会影响动物的毒力，但其对植物病原体的影响尚不清楚。此前，我们发现植物病原体丁香假单胞菌的III型分泌系统（T3SS）在植物体内表现出表型变异。在此，我们使用流式细胞术和显微镜来研究单细胞鞭毛表达与T3SS表达的关系，结果表明，在模仿质外体的培养基中以及在菜豆整个定殖过程中的质外体微菌落内，这两个系统在体外均表现出表型异质性。随机、空间和时间因素塑造了一个表型多样的病原体群体的动态，该群体在定殖过程中表现出分工：表达T3SS的细菌产生的效应子作为“公共物品”来抑制免疫，使表达运动性鞭毛的细菌能够增加并在坏死前离开受感染组织。这些结果展示了在一个与环境和农业相关的系统中，植物定殖过程中细菌专业化的机制。

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