Yang Chunmei, Li Lihong, Li Mingdong, Shu Yue, Luo Yiping, Gu Didi, Zhu Xin, Chen Jing, Yang Lu, Shu Jian
Department of Radiology, The Affiliated Hospital of Southwest Medical University, Precision Imaging and Intelligent Analysis Key Laboratory of Luzhou, Luzhou, Sichuan, 646000, China.
Department of Oncology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, 646000, China.
Nano Converg. 2025 Apr 2;12(1):18. doi: 10.1186/s40580-025-00483-4.
Triple negative breast cancer (TNBC) remains a challenge for clinical diagnosis and therapy due to its poor prognosis and high mortality rate. Hence, new methods to achieve TNBC imaging and imaging-guided TNBC therapy are urgently needed. Currently, the combination of chemotherapy with phototherapy/catalytic therapy has become a promising strategy for cancer treatment. Here, multifunctional CuFeSe ternary nanozymes (CuFeSe-AMD3100-Gem nanosheets) were prepared as high-performance nanotheranostic agents for imaging-guided synergistic therapy of TNBC in vitro and in vivo. CuFeSe-AMD3100-Gem nanosheets not only exhibited outstanding CXCR4-targeted capability and superior photothermal properties, but also produced exact chemical cytotoxicity through the loading of the chemotherapy drug Gemcitabine. Specifically, the CuFeSe-AMD3100-Gem nanosheets simultaneously possessed peroxidase-like activities capable of converting endogenous HO to hydroxyl radicals (•OH), which could be significantly enhanced under light irradiation. Furthermore, these nanosheets showed remarkable multimodal imaging ability for magnetic resonance imaging (MRI), computed tomography (CT) and infrared thermography in TNBC tumor-bearing mice (4T1 cells). More importantly, the in vitro and in vivo results verified the significant synergistic anticancer effect of the CuFeSe-AMD3100-Gem nanosheets by combining photothermal therapy and enzyme catalytic therapy with chemotherapy. In conclusion, these advantages demonstrate the powerful potential of CuFeSe ternary nanozymes for imaging-guided synergistic photothermal/catalytic/chemical therapy for TNBC.
三阴性乳腺癌（TNBC）因其预后差和死亡率高，仍然是临床诊断和治疗面临的挑战。因此，迫切需要实现TNBC成像及成像引导下TNBC治疗的新方法。目前，化疗与光疗/催化治疗相结合已成为一种很有前景的癌症治疗策略。在此，制备了多功能铜铁硒三元纳米酶（CuFeSe-AMD3100-Gem纳米片）作为高性能纳米诊疗剂，用于在体外和体内对TNBC进行成像引导的协同治疗。CuFeSe-AMD3100-Gem纳米片不仅表现出出色的CXCR4靶向能力和优异的光热性能，还通过负载化疗药物吉西他滨产生确切的化学细胞毒性。具体而言，CuFeSe-AMD3100-Gem纳米片同时具有类过氧化物酶活性，能够将内源性H₂O₂转化为羟基自由基（•OH），在光照下可显著增强。此外，这些纳米片对荷TNBC肿瘤小鼠（4T1细胞）的磁共振成像（MRI）、计算机断层扫描（CT）和红外热成像显示出显著的多模态成像能力。更重要的是，体外和体内实验结果证实了CuFeSe-AMD3100-Gem纳米片通过将光热疗法、酶催化疗法与化疗相结合，具有显著的协同抗癌效果。总之，这些优势证明了铜铁硒三元纳米酶在成像引导下对TNBC进行协同光热/催化/化学治疗方面具有强大的潜力。
