Roberts J E, Roy D, Dillon J
Curr Eye Res. 1985 Mar;4(3):181-5. doi: 10.3109/02713688509000848.
Hematoporphyrin (HP), a drug used for the treatment of tumors including intraocular tumors, is an efficient photosensitizer. In addition to its therapeutic value, it also produces a phototoxic side effect in the skin. To test whether such effects may also occur in the eye, calf lens fiber membranes were photolyzed in the presence and absence of 1 mM HP. A marked increase (ca 5 times) in the photopolymerization of the calf lens membrane main intrinsic protein (MP26) was found in the presence of HP. Tenfold increases in destruction rates were found in losses of histidine. The MP26 was also photolyzed after tryptic and chymotryptic digestion to MP21, this resulted in an increased photopolymerization in the presence of 1 mM HP. These data suggest an age related increase in sensitivity of the lens fiber membrane proteins to such photoprocesses. The addition of both azide and penicillamine reduces the photosensitized loss of the main intrinsic protein.
血卟啉（HP）是一种用于治疗包括眼内肿瘤在内的肿瘤的药物，是一种有效的光敏剂。除了其治疗价值外，它还会在皮肤中产生光毒性副作用。为了测试这种效应是否也会在眼睛中发生，在有和没有1 mM HP的情况下对小牛晶状体纤维膜进行光解。在有HP的情况下，发现小牛晶状体膜主要内在蛋白（MP26）的光聚合有显著增加（约5倍）。组氨酸损失的破坏率增加了10倍。MP26在经胰蛋白酶和糜蛋白酶消化成MP21后也被光解，这导致在1 mM HP存在下光聚合增加。这些数据表明晶状体纤维膜蛋白对这种光过程的敏感性随年龄增长而增加。叠氮化物和青霉胺的添加均可减少主要内在蛋白的光致敏损失。
