Dillon J, Roy D, Spector A
Exp Eye Res. 1985 Jul;41(1):53-60. doi: 10.1016/0014-4835(85)90093-4.
Calf lens fiber membranes were photolyzed in the presence and absence of sensitizers and scavengers. Photolytic damage was assessed by SDS-polyacrylamide gel electrophoresis, UV and fluorescence spectra and amino acid analyses. With irradiation, there is an apparent polymerization of the major membrane polypeptide (MP26) and the formation of material which does not enter SDS-polyacrylamide gels. Some degradation was also observed. These changes are accompanied by losses of histidine and tryptophan and changes in the UV spectra. The rate of photolysis is enhanced in the presence of the glucoside of 3-hydroxykynurenine (3-OH-KYN), a compound endogenous to the lens. The reaction is retarded in the presence of sulfhydryl-containing compounds such as glutathione.
在有和没有敏化剂及清除剂的情况下对小牛晶状体纤维膜进行光解。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、紫外光谱和荧光光谱以及氨基酸分析来评估光解损伤。随着辐照，主要膜多肽（MP26）明显聚合，并且形成了不进入十二烷基硫酸钠-聚丙烯酰胺凝胶的物质。还观察到了一些降解。这些变化伴随着组氨酸和色氨酸的损失以及紫外光谱的改变。在3-羟基犬尿氨酸（3-OH-KYN）的糖苷（晶状体的一种内源性化合物）存在的情况下，光解速率会提高。在含有巯基的化合物（如谷胱甘肽）存在时，反应会受到抑制。
