Veit B C, Jones J M, Miller G A, Feldman J D
Int J Cancer. 1977 Jan;19(1):97-106. doi: 10.1002/ijc.2910190114.
Brown Norway (BN), Lewis (Le), F1 hybrids of LexBN (LBN) and parent-to-LBN backcross rats were tested for cellular and humoral responses to a BN Moloney sarcoma. Regardless of AgB phenotype, BN backcrosses produced low levels of cell-mediated cytotoxicity (CMC) that were comparable to those of BN parents. Le backcrosses developed high levels of CMC similar to those produced in Le parents. An inverse relationship between levels of CMC and serum antibodies (cytotoxic for tumor cells and anti-p30 of MuLV) was observed; BN parents and backcrosses produced high levels of serum antibodies whereas levels in Le parents and backcrosses were low. LBN hybrids developed relatively high levels of CMC and serum antibodies. An additional finding was that the CMC response in Le parents and back-crosses was directed primarily against tumor-associated antigens rather than histocompatability antigens expressed on the tumor cells. The results suggest that humoral and cellular responses to Moloney sarcoma in rats are not determined solely by the major AgB histocompatibility locus but do have a genetic association. This genetic association was detected with a 51Cr release assay which detects T-cells, suggesting that select populations of effector T-cells may be genetically regulated.
对棕色挪威（BN）大鼠、刘易斯（Le）大鼠、LexBN（LBN）的F1杂种大鼠以及亲代与LBN的回交大鼠进行了检测，观察它们对BN莫洛尼肉瘤的细胞和体液反应。无论AgB表型如何，BN回交大鼠产生的细胞介导细胞毒性（CMC）水平较低，与BN亲代相当。Le回交大鼠产生的CMC水平较高，与Le亲代相似。观察到CMC水平与血清抗体（对肿瘤细胞具有细胞毒性以及抗鼠白血病病毒p30）之间呈负相关；BN亲代和回交大鼠产生高水平的血清抗体，而Le亲代和回交大鼠中的水平较低。LBN杂种大鼠产生相对较高水平的CMC和血清抗体。另一个发现是，Le亲代和回交大鼠中的CMC反应主要针对肿瘤相关抗原，而非肿瘤细胞上表达的组织相容性抗原。结果表明，大鼠对莫洛尼肉瘤的体液和细胞反应并非仅由主要的AgB组织相容性基因座决定，但确实存在遗传关联。通过检测T细胞的51Cr释放试验检测到了这种遗传关联，这表明效应T细胞的特定群体可能受到基因调控。
