Epstein L B, Knight R A
Br J Cancer. 1975 May;31(5):499-512. doi: 10.1038/bjc.1975.90.
Antigenic determinants of p30, the most abundant internal virion protein of C type RNA viruses, were detected on the surface of spleen cells from mice bearing Moloney leukaemia and on an in vitro line of Moloney sarcoma, MSC. On both cell types, these determinants on the p30 molecules served as cytotoxic targets in a xenogenic complement dependent antibody mediated 51Cr release assay. Two antisera were used: a rat anti MLV -M induced lymphoma serum, and an antiserum raised in goats to either disrupted FeLV. The cytotoxic target antigens of these antisera were analysed by inhibition of cytotoxicity with viral and cellular proteins.
p30是C型RNA病毒中最丰富的内部病毒粒子蛋白,在患有莫洛尼白血病的小鼠脾脏细胞表面以及莫洛尼肉瘤体外细胞系MSC上检测到了其抗原决定簇。在这两种细胞类型上,p30分子上的这些抗原决定簇在异种补体依赖性抗体介导的51Cr释放试验中作为细胞毒性靶标。使用了两种抗血清:一种大鼠抗MLV -M诱导的淋巴瘤血清,以及一种用山羊制备的针对灭活猫白血病病毒的抗血清。通过用病毒蛋白和细胞蛋白抑制细胞毒性来分析这些抗血清的细胞毒性靶标抗原。