Karimian Tina, Lanzerstorfer Peter, Weghuber Julian
University of Applied Sciences Upper Austria, Center of Excellence Food Technology and Nutrition, Stelzhamerstrasse 23, 4600, Wels, Austria.
FFoQSI GmbH-Austrian Competence Centre for Feed and Food Quality, Safety and Innovation, Stelzhamerstrasse 23, 3430, Tulln, Austria.
Mater Today Bio. 2025 Mar 17;32:101672. doi: 10.1016/j.mtbio.2025.101672. eCollection 2025 Jun.
Soft lithography-based contact printing techniques have evolved into versatile methods for creating micro- and nanoscale features of biomolecules on solid substrates. In this review we present the advances in soft lithography for biomolecule deposition and its applications in subcellular protein-protein interaction (PPI) analysis. We discuss various soft lithography techniques, including micro-contact printing (μCP), nano-contact printing (nCP), capillary nanostamping, and polymer-pen-lithography (PPL) and focus on their application in biomolecule patterning on diverse substrates. We then address related challenges and advancements, including substrate selection, surface activation methods, and stamp development. The specific advantages, limitations, and potential solutions for printing various inks and biomolecules are highlighted. Furthermore, recent advances in soft lithography-based biomolecule patterning for subcellular protein interaction analysis are emphasized, demonstrating the importance of these techniques for incorporating complex cellular events into PPI readout modalities and established protein deposition strategies. Finally, an overview of future technologies and enhanced applications is provided.
基于软光刻的接触印刷技术已发展成为在固体基质上创建生物分子微纳尺度特征的通用方法。在本综述中，我们介绍了用于生物分子沉积的软光刻技术的进展及其在亚细胞蛋白质 - 蛋白质相互作用（PPI）分析中的应用。我们讨论了各种软光刻技术，包括微接触印刷（μCP）、纳米接触印刷（nCP）、毛细管纳米压印和聚合物笔光刻（PPL），并重点介绍了它们在不同基质上生物分子图案化中的应用。然后，我们探讨了相关的挑战和进展，包括基质选择、表面活化方法和印章开发。突出了印刷各种油墨和生物分子的具体优势、局限性及潜在解决方案。此外，还强调了基于软光刻的生物分子图案化在亚细胞蛋白质相互作用分析方面的最新进展，证明了这些技术对于将复杂细胞事件纳入PPI读出模式和既定蛋白质沉积策略的重要性。最后，提供了未来技术和增强应用的概述。
