Utami Diah Tri
Department of Pharmacy, Faculty of Medicine and Health Sciences, University of Jambi, Jambi, Indonesia.
Department of Chemistry, Faculty of Science and Technology, University of Jambi, Jambi, Indonesia.
J Adv Pharm Technol Res. 2025 Jan-Mar;16(1):18-23. doi: 10.4103/JAPTR.JAPTR_222_24. Epub 2025 Feb 14.
In traditional medicine, has been used for its many bioactive components that are good for people's health. This research assessed the cytotoxic effects of extracts and fractions against Michigan Cancer Foundation-7 (MCF-7) and Henrietta Lacks (HeLa) cell lines. The extracts and fractions of root and fruit assess cytotoxic activities and inhibitory mechanisms against the MCF-7 and HeLa cancer cell lines, respectively. The fruit and roots of were extracted using the liquid-liquid method. The sample concentrations evaluated included extracts 31.5-1000 μg/mL, fractions 15.625-500 μg/mL, and doxorubicin 2-0.0625 μg/mL. Cytotoxicity was evaluated on MCF-7 and HeLa cells using an MTT assay. Morphological alterations were subsequently discovered utilizing an inverted microscope. Flow cytometry was utilized to find out the cell cycle's distribution and the apoptosis characteristics. The different parts and extracts showed cytotoxic effects on HeLa and MCF-7 cells, with IC values ranging from 30.69 ± 0.47 to 787.89 ± 1.77 µg/mL. Cell cycle studies showed that fraction A4 inhibited the cell cycle in MCF-7 cells at the G1 phase followed by the G2/M and S phases, while fraction B5 inhibited the cell cycle in HeLa cells at the G2/M phase. Both fractions showed the ability to induce apoptosis against MCF-7 and HeLa cells. The results showed that the fractions A4 and B5 showed cytotoxic activity against MCF-7 and HeLa cells by cell cycle arrest and apoptosis induction.
在传统医学中，因其多种对人体健康有益的生物活性成分而被使用。本研究评估了提取物和馏分对密歇根癌症基金会 -7（MCF -7）和海瑞塔·拉克斯（HeLa）细胞系的细胞毒性作用。根和果实的提取物及馏分分别评估了对MCF -7和HeLa癌细胞系的细胞毒性活性及抑制机制。采用液 - 液法提取了[植物名称未给出]的果实和根。评估的样品浓度包括提取物31.5 - 1000μg/mL、馏分15.625 - 500μg/mL以及阿霉素2 - 0.0625μg/mL。使用MTT法评估MCF -7和HeLa细胞的细胞毒性。随后利用倒置显微镜发现形态学改变。运用流式细胞术确定细胞周期分布及凋亡特征。不同部位和提取物对HeLa和MCF -7细胞显示出细胞毒性作用，IC值范围为30.69±0.47至787.89±1.77μg/mL。细胞周期研究表明，馏分A4在G1期抑制MCF -7细胞的细胞周期，随后是G2/M期和S期，而馏分B5在G2/M期抑制HeLa细胞的细胞周期。两种馏分均显示出对MCF -7和HeLa细胞诱导凋亡的能力。结果表明，馏分A4和B5通过细胞周期阻滞和诱导凋亡对MCF -7和HeLa细胞显示出细胞毒性活性。
