Chang Jiaxing, Zhang Zhinan, Qu Chulin, Han Qingzhi, Xu Li
College of Science, Nanjing Forestry University, Nanjing 210037, China.
State Key Laboratory of Coordination Chemistry, Collaborative Innovation Center of Advanced Microstructures, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Anal Chem. 2025 Apr 15;97(14):7842-7850. doi: 10.1021/acs.analchem.4c06431. Epub 2025 Apr 3.
We report a strategy based on pyridyl-anchored organic small-molecule fluorescent probes to develop a dual-signal sensing platform. The strategy accomplishes an intelligent integration of fluorescence analysis with photoelectrochemical (PEC) sensing, thereby enabling rapid and precise detection of hypochlorite. In this work, the natural dye chromone was selected as the fluorophore for generating fluorescent signals. Meanwhile, by using phenothiazine (PTZ) as the specific recognition group and pyridine as the anchoring moiety, we designed and synthesized a novel organic small-molecule fluorescent probe. The obtained probe was used as a photosensitive material anchored to the TiO surface via N → Ti bonds, to form an FTO/TiO/FPTZ-1 heterostructure-based dual-signal sensing platform for the detection of hypochlorite. This sensing platform has the characteristics of high specificity, sensitivity, and ease of preparation, enabling rapid qualitative fluorescence readout and quantitative photoelectrochemical readout of hypochlorite, with a limit of detection of 0.288 μM for fluorescence and 1.37 nM for PEC.
我们报道了一种基于吡啶基锚定有机小分子荧光探针的策略，以开发一种双信号传感平台。该策略实现了荧光分析与光电化学（PEC）传感的智能整合，从而能够快速、精确地检测次氯酸盐。在这项工作中，选择天然染料色酮作为产生荧光信号的荧光团。同时，以吩噻嗪（PTZ）作为特异性识别基团，吡啶作为锚定部分，设计并合成了一种新型有机小分子荧光探针。将所得探针用作通过N→Ti键锚定在TiO表面的光敏材料，形成基于FTO/TiO/FPTZ-1异质结构的双信号传感平台用于检测次氯酸盐。该传感平台具有高特异性、高灵敏度和易于制备的特点，能够实现次氯酸盐的快速定性荧光读出和定量光电化学读出，荧光检测限为0.288μM，PEC检测限为1.37 nM。
