Gong Pengfei, Gao Zehang, Sun Rui, He Binghuan, Yuan Fei, Chen Chang, Zhao Jianlong, Su Hengxiang, Wang Lihua, Liu Bo, Feng Shilun
Jiaxing Key Laboratory of Biosemiconductors, Xiangfu Laboratory, Jiashan, Zhejiang, 314102, China; State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.
Talanta. 2025 Oct 1;293:128005. doi: 10.1016/j.talanta.2025.128005. Epub 2025 Mar 27.
Rapid and sensitive detection of Escherichia coli (E. coli) is crucial for ensuring water safety. Traditional culture-based detection technologies are time-consuming, cumbersome, and difficult to meet immediate needs, while existing instrument-based detection is costly, time-consuming, and struggle to process complex samples. Herein, a microfluidic system for rapid enrichment and sensitive detection of E. coli in water based on bilayer membrane and high flux droplets is proposed. The bilayer membrane structure can enrich bacteria while removing impurities in the sample. High-throughput droplets can encapsulate single bacteria for enzymatic reactions within the droplets, reducing the reaction system to nanoliter, and greatly shortening the reaction time. The results showed that the detection of E. coli solution with different concentrations by this system had little difference compared to the plate counting method, demonstrating a good linear relationship (R = 0.9968) and the detection of total coliform in sewage has an accuracy rate of at least 80 %. In summary, this system combines enrichment technology with microdroplet technology to detect E. coli for the first time. It can achieve 50-fold enrichment of E. coli in water and bacteria isolation in 100 μL solution with tens of thousands of droplets, which greatly improves the sensitivity and efficiency of detection, thus showing great potential for ensuring water safety.
快速灵敏地检测大肠杆菌对于确保水安全至关重要。传统的基于培养的检测技术耗时、繁琐，难以满足即时需求，而现有的基于仪器的检测成本高、耗时，且难以处理复杂样本。在此，提出了一种基于双层膜和高通量液滴的用于水中大肠杆菌快速富集和灵敏检测的微流控系统。双层膜结构可以富集细菌，同时去除样本中的杂质。高通量液滴可以封装单个细菌以在液滴内进行酶促反应，将反应体系缩小至纳升，大大缩短反应时间。结果表明，该系统对不同浓度大肠杆菌溶液的检测与平板计数法相比差异不大，呈现出良好的线性关系（R = 0.9968），并且对污水中总大肠菌群的检测准确率至少为80%。综上所述，该系统首次将富集技术与微滴技术相结合用于检测大肠杆菌。它可以在水中实现大肠杆菌50倍的富集，并在100 μL溶液中通过数以万计的液滴实现细菌分离，大大提高了检测的灵敏度和效率，从而在确保水安全方面显示出巨大潜力。
