Su Xi, Ge Chuang, Xiang Songtao, Wu Xiaoqin, Wang Yipei, Chen Li, Xu Yi
School of Advanced Materials Engineering, Jiaxing Nanhu University, Jiaxing, 314001, People's Republic of China.
Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Key Laboratory of Optoelectronic Technology and Systems, Ministry of Education, Chongqing University, Shapingba District, No. 174, St. Shazhengjie, Chongqing, 400044, People's Republic of China.
Mikrochim Acta. 2025 Sep 12;192(10):664. doi: 10.1007/s00604-025-07539-6.
The detection of surface microbial contaminants faces several critical challenges, including disconnection between sampling and detection steps and time-consuming. To overcome these limitations, we developed an innovative integrated microfluidic system that combines bacterial sampling, transfer, concentration, and detection within a single chip platform. The microchip architecture consists of a thermos-responsive flexible sheet incorporating poly(N-isopropylacrylamide) (PNIPAAm) gel micropillar arrays for efficient bacterial capture and thermal-triggered release, and a PDMS cover layer with an optimized canopy-shaped microchannel design for sequential bacterial transport, fluorescent labeling, and in situ concentration/fluorescence detection. To verify the system's functionality, Staphylococcus aureus (S. aureus) was employed as a model organism for testing the microchip detection platform. Under optimized conditions, the sampling and the elution efficiency of S. aureus on stainless steel sheet were up to 75.6% and 88.4%, respectively. The detection limit of S. aureus was 5.15 × 10 CFU/cm, and the entire process was completed in < 45 min. This integrated microfluidic system performs continuous surface microbial sampling and detection, reducing processing time by 32-fold compared to conventional swab-based methods. The platform offers a rapid, integrated, and highly efficient solution for bacterial detection for surface microbial contaminants, with promising applications in food safety, medical hygiene, and environmental monitoring.
表面微生物污染物的检测面临着几个关键挑战，包括采样与检测步骤之间的脱节以及耗时问题。为了克服这些限制，我们开发了一种创新的集成微流控系统，该系统在单个芯片平台内结合了细菌采样、转移、浓缩和检测功能。微芯片架构由一个包含聚（N-异丙基丙烯酰胺）（PNIPAAm）凝胶微柱阵列的热响应柔性片组成，用于高效捕获细菌并通过热触发释放，以及一个带有优化的伞形微通道设计的聚二甲基硅氧烷（PDMS）覆盖层，用于细菌的顺序运输、荧光标记以及原位浓缩/荧光检测。为了验证该系统的功能，以金黄色葡萄球菌作为模式生物来测试微芯片检测平台。在优化条件下，金黄色葡萄球菌在不锈钢片上的采样和洗脱效率分别高达75.6%和88.4%。金黄色葡萄球菌的检测限为5.15×10 CFU/cm，整个过程在不到45分钟内完成。这种集成微流控系统可进行连续的表面微生物采样和检测，与传统的基于拭子的方法相比，处理时间减少了32倍。该平台为表面微生物污染物的细菌检测提供了一种快速、集成且高效的解决方案，在食品安全、医疗卫生和环境监测方面具有广阔的应用前景。
