噬菌体T4的基因55、α gt、47和46：通过序列分析推导的基因组组织
Genes 55, alpha gt, 47 and 46 of bacteriophage T4: the genomic organization as deduced by sequence analysis.
作者信息
Gram H, Rüger W
出版信息
EMBO J. 1985 Jan;4(1):257-64. doi: 10.1002/j.1460-2075.1985.tb02344.x.
Abstract
The nucleotide sequence of T4 genes 55, alpha gt, 47 and 46 was determined by a combination of 'classical' procedures and a shotgun approach. Small DNA fragments generated by frequent cleavage with restriction enzymes or by sonication of restriction fragments were cloned in phage M13 vectors and sequenced by the dideoxy method. The positions of the genes were determined by marker rescue between the corresponding T4 amber mutants and the cloned T4 DNA fragments used in the sequencing experiments. The sequence gives an insight into the organization of this 7.1-kb early region of the T4 genome and shows that genetically 'silent' portions within this region are not void of genetic information.
摘要
通过“经典”方法与鸟枪法相结合，确定了T4基因55、α gt、47和46的核苷酸序列。用限制性内切酶频繁切割或对限制性片段进行超声处理产生的小DNA片段，克隆到噬菌体M13载体中，并通过双脱氧法进行测序。基因的位置通过相应的T4琥珀突变体与测序实验中使用的克隆T4 DNA片段之间的标记拯救来确定。该序列揭示了T4基因组这个7.1 kb早期区域的组织情况，并表明该区域内遗传上“沉默”的部分并非没有遗传信息。
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1
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Nucleic Acids Res. 1980 Aug 25;8(16):3673-94. doi: 10.1093/nar/8.16.3673.
2
Genetic and amber fragment maps of genes 46 and 47 of bacteriophage T4D.噬菌体T4D基因46和47的遗传图谱和琥珀片段图谱
J Virol. 1981 Oct;40(1):309-13. doi: 10.1128/JVI.40.1.309-313.1981.
3
The making of strand-specific M13 probes.链特异性M13探针的制备。
Gene. 1982 Mar;17(3):271-7. doi: 10.1016/0378-1119(82)90143-3.
4
Use of the 'Perceptron' algorithm to distinguish translational initiation sites in E. coli.使用“感知器”算法区分大肠杆菌中的翻译起始位点。
Nucleic Acids Res. 1982 May 11;10(9):2997-3011. doi: 10.1093/nar/10.9.2997.
5
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7
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