Fang Po-Hsiang, Chen Guan-Xu, Wang Shuying, Teng Ching-Hao, Huang Wen-Chun, Cheng Horng-Long, Chou Wei-Yang
Department of Photonics, National Cheng Kung University, Tainan 70101, Taiwan.
Department of Microbiology and Immunology, Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan.
ACS Appl Bio Mater. 2025 Apr 21;8(4):2867-2874. doi: 10.1021/acsabm.4c01618. Epub 2025 Apr 3.
In the realms of modern medicine and environmental monitoring, there is an escalating demand for bacterial detection technologies that are rapid, precise, and highly sensitive. Conventional methods, however, are often hindered by their time-intensive nature, procedural complexity, and reliance on specialized laboratory equipment. This study introduces an innovative approach utilizing bovine serum albumin (BSA) as the dielectric layer and lysozyme (LYZ) as the bacterial sensing layer in organic field-effect transistors (OFETs). The combination of BSA and LYZ enhances both biocompatibility and detection sensitivity, enabling precise differentiation between Gram-positive and Gram-negative bacteria. BSA not only stabilizes the electrical performance of the OFET but also offers biodegradability and water solubility, contributing to environmental sustainability. These biocompatible OFETs can accurately detect bacterial concentrations ranging from 10 to 10 CFU/mL, with real-time response capabilities via multispike measurements. This research represents a significant step forward in the development of advanced, portable biosensors for use in complex biological environments, advancing bacterial detection technology.
在现代医学和环境监测领域，对快速、精确且高度灵敏的细菌检测技术的需求不断升级。然而，传统方法常常因其耗时的特性、程序复杂性以及对专业实验室设备的依赖而受到阻碍。本研究引入了一种创新方法，在有机场效应晶体管（OFET）中利用牛血清白蛋白（BSA）作为介电层，溶菌酶（LYZ）作为细菌传感层。BSA和LYZ的结合增强了生物相容性和检测灵敏度，能够精确区分革兰氏阳性菌和革兰氏阴性菌。BSA不仅稳定了OFET的电学性能，还具有生物可降解性和水溶性，有助于环境可持续性。这些生物相容性OFET能够准确检测10至10 CFU/mL范围内的细菌浓度，并通过多次加样测量具备实时响应能力。这项研究代表了用于复杂生物环境的先进便携式生物传感器开发方面的重要进展，推动了细菌检测技术的发展。
