Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Cancer Metabolism and Epigenetic Unit, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Centre of Artificial Intelligence in Precision Medicines, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Nano Diagnostics & Devices (NDD), IT Medical Fusion Center, 350-27 Gumidae-ro, Gumi-si, Gyeongbuk 39253, Republic of Korea.
Bioelectrochemistry. 2022 Feb;143:107982. doi: 10.1016/j.bioelechem.2021.107982. Epub 2021 Oct 15.
The large-scale diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important for traceability and treatment during pandemic outbreaks. We developed a fast (2-3 min), easy-to-use, low-cost, and quantitative electrochemical biosensor based on carbon nanotube field-effect transistor (CNT-FET) that allows digital detection of the SARS-CoV-2 S1 in fortifited saliva samples for quick and accurate detection of SARS-CoV-2 S1 antigens. The biosensor was developed on a Si/SiO surface by CNT printing with the immobilization of a anti-SARS-CoV-2 S1. SARS-CoV-2 S1 antibody was immobilized on the CNT surface between the S-D channel area using a linker 1-pyrenebutanoic acid succinimidyl ester (PBASE) through non-covalent interaction. A commercial SARS-CoV-2 S1 antigen was used to characterize the electrical output of the CNT-FET biosensor. The SARS-CoV-2 S1 antigen in the 10 mM AA buffer pH 6.0 was effectively detected by the CNT-FET biosensor at concentrations from 0.1 fg/mL to 5.0 pg/mL. The limit of detection (LOD) of the developed CNT-FET biosensor was 4.12 fg/mL. The selectivity test was performed by using target SARS-CoV-2 S1 and non-target SARS-CoV-1 S1 and MERS-CoV S1 antigens in the 10 mM AA buffer pH 6.0. The biosensor showed high selectivity (no response to SARS-CoV-1 S1 or MERS-CoV S1 antigen) with SARS-CoV-2 S1 antigen detection in the 10 mM AA buffer pH 6.0. The biosensor is highly sensitive, saves time, and could be a helpful platform for rapid detection of SARS-CoV-2 S1 antigen from the patients saliva.
大规模诊断严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）对于大流行期间的溯源和治疗非常重要。我们开发了一种基于碳纳米管场效应晶体管（CNT-FET）的快速（2-3 分钟）、易用、低成本和定量电化学生物传感器，可对强化唾液样本中的 SARS-CoV-2 S1 进行数字检测，从而快速准确地检测 SARS-CoV-2 S1 抗原。该生物传感器是在 Si/SiO 表面上通过 CNT 打印制成的，其中固定有抗 SARS-CoV-2 S1。SARS-CoV-2 S1 抗体通过非共价相互作用用 1-蒽丁酸琥珀酰亚胺酯（PBASE）固定在 CNT 表面上的 S-D 通道区域之间。使用商业 SARS-CoV-2 S1 抗原来表征 CNT-FET 生物传感器的电输出。SARS-CoV-2 S1 抗原在 10mM AA 缓冲液 pH 6.0 中的浓度从 0.1fg/mL 到 5.0pg/mL 时，可以被 CNT-FET 生物传感器有效检测。所开发的 CNT-FET 生物传感器的检测限（LOD）为 4.12fg/mL。通过在 10mM AA 缓冲液 pH 6.0 中使用靶标 SARS-CoV-2 S1 和非靶标 SARS-CoV-1 S1 和 MERS-CoV S1 抗原进行选择性测试。该生物传感器对 SARS-CoV-2 S1 抗原表现出高选择性（对 SARS-CoV-1 S1 或 MERS-CoV S1 抗原无响应），在 10mM AA 缓冲液 pH 6.0 中检测 SARS-CoV-2 S1 抗原。该生物传感器具有高灵敏度，节省时间，并且可以成为从患者唾液中快速检测 SARS-CoV-2 S1 抗原的有用平台。
