Rahman M S, Mettrick D F, Podesta R B
Exp Parasitol. 1985 Aug;60(1):10-7. doi: 10.1016/s0014-4894(85)80018-7.
The effect of Serotonin on carbohydrate metabolism, excreted end products, and adenine nucleotide pools in Schistosoma mansoni was determined following 60 min in vitro incubations under air (= 21% O2) and anaerobic (95% N2:5% CO2) conditions. In the presence of 0.25 mM Serotonin, glucose uptake increased by 82-84% and lactate excretion increased by 77-78%; levels of excreted lactate were significantly higher under aerobic than under anaerobic conditions. The tissue pools of glucose, hexosephosphates, fructose 1,6-bisphosphate, pyruvate, and lactate were significantly increased under anaerobic conditions compared to air incubation; the presence of Serotonin decreased tissue glucose pools and increased the size of the pyruvate and lactate tissue pools. The glycolytic carbon pool was significantly greater under anaerobic than under aerobic conditions, irrespective of Serotonin. Serotonin increased adenosine 5'-diphosphate and adenosine 5'-monophosphate levels under aerobic conditions; neither Serotonin nor gas phase significantly affected total adenine nucleotide levels or the adenylate energy charge. Serotonin increased energy requirements by S. mansoni due to increased muscle contractions; demand was met by enhanced rates of carbohydrate metabolism. Irrespective of gas phase, 74-78% of available carbohydrate was converted to lactate. In the presence of Serotonin, conversion of glucose to lactate was reduced to 63-67%. In view of the requirements by S. mansoni for an abundant supply of glycoprotein and glycolipid precursors for surface membrane renewal, it is suggested that carbohydrate (glucose and glycogen) that was not converted to lactate may have been incorporated into biosynthetic processes leading to membrane synthesis.
在空气（=21% O₂）和厌氧（95% N₂:5% CO₂）条件下进行60分钟体外孵育后，测定了血清素对曼氏血吸虫碳水化合物代谢、排泄终产物和腺嘌呤核苷酸库的影响。在存在0.25 mM血清素的情况下，葡萄糖摄取增加了82 - 84%，乳酸排泄增加了77 - 78%；有氧条件下排泄的乳酸水平显著高于厌氧条件下。与空气孵育相比，厌氧条件下葡萄糖、己糖磷酸、果糖1,6 - 二磷酸、丙酮酸和乳酸的组织库显著增加；血清素的存在降低了组织葡萄糖库，增加了丙酮酸和乳酸组织库的大小。无论有无血清素，厌氧条件下糖酵解碳库均显著大于有氧条件下。有氧条件下血清素增加了腺苷5'-二磷酸和腺苷5'-单磷酸水平；血清素和气相均未显著影响总腺嘌呤核苷酸水平或腺苷酸能量电荷。血清素由于肌肉收缩增加而增加了曼氏血吸虫的能量需求；碳水化合物代谢速率的提高满足了这种需求。无论气相如何，74 - 78%的可用碳水化合物转化为乳酸。在存在血清素的情况下，葡萄糖向乳酸的转化率降至63 - 67%。鉴于曼氏血吸虫需要大量糖蛋白和糖脂前体来更新表面膜，有人提出未转化为乳酸的碳水化合物（葡萄糖和糖原）可能已被纳入导致膜合成的生物合成过程中。
