Cheng Jie, Tan Long, Wang Yucai, Gao Mengwei, Liu Feifei, Wang Qi, Xu Chengshuai, Zhang Chaobo, Xu Wei, Hou Yuyong, Jiang Tong, Zhao Lei
State Key Laboratory of Macromolecular Drugs and Large-scale Preparation, School of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng, China.
State Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
Front Microbiol. 2025 Mar 13;16:1540631. doi: 10.3389/fmicb.2025.1540631. eCollection 2025.
residue has attracted increasing attention owing to its antibacterial effect, which presents potential as novel antimicrobial agents for the management of algal blooms. In this study, the ability of residue extract to control algal blooms has been firstly verified. Then, the response surface methodology was employed to optimize flavonoids yield, the primary antimicrobial component in extract, and the underlying photosynthetic inhibition mechanisms of extract on was investigated. Results showed that the highest yield of total flavonoids was increased to 4.08% when the extraction conditions were a liquid-to-solid ratio of 10:1, ethanol concentration of 60%, extraction temperature of 80°C, and extraction time of 30 min. Meanwhile, treatment with residue extract at doses of 40.0 mg/L significantly decreased the Fv/Fm and PIabs values of by 24.36 and 88.87% at 50 h, respectively. The added extract induced damage at multiple levels of physiological and biochemical processes in algal cells, including reduced electron transport capability, disrupted energy transfer in photosystem II, disruption of OEC structure, and altered energy distribution in PSII reaction center. To our knowledge, this study was the first verification of residue's algicidal potential, and these findings in our study contribute to a deeper understanding of the allelopathic mechanisms of residue extract and offer valuable insights for the management of algal blooms.
残留物因其抗菌作用而受到越来越多的关注，这使其作为治理藻华的新型抗菌剂具有潜力。在本研究中，首次验证了残留物提取物控制藻华的能力。然后，采用响应面法优化提取物中主要抗菌成分黄酮类化合物的产量，并研究了提取物对[具体藻类名称未给出]的潜在光合抑制机制。结果表明，当提取条件为液固比10:1、乙醇浓度60%、提取温度80°C和提取时间30分钟时，总黄酮的最高产量提高到了4.08%。同时，在50小时时，以40.0 mg/L剂量的[具体藻类名称未给出]残留物提取物处理显著降低了[具体藻类名称未给出]的Fv/Fm和PIabs值，分别降低了24.36%和88.87%。添加的提取物在藻类细胞的多个生理生化过程水平上造成了损害，包括电子传递能力降低、光系统II中的能量传递中断、放氧复合体结构破坏以及PSII反应中心的能量分布改变。据我们所知，本研究首次验证了[具体藻类名称未给出]残留物的杀藻潜力，我们的这些研究结果有助于更深入地理解[具体藻类名称未给出]残留物提取物的化感作用机制，并为藻华治理提供有价值的见解。
