Dale B, DeFelice L J, Ehrenstein G
Experientia. 1985 Aug 15;41(8):1068-70. doi: 10.1007/BF01952148.
Fertilization membranes form around unfertilized sea-urchin eggs after microinjection of a soluble spermatozoa fraction isosmotic with seawater. This demonstrates that the spermatozoon contains a chemical that triggers an increase in cytosolic calcium, leading to exocytosis of cortical granules. It also demonstrates that the triggering mechanism does not require an externally-activated egg-membrane process. Further experiments show that the chemical trigger is not calcium.
在用与海水等渗的可溶性精子部分进行显微注射后，未受精的海胆卵周围会形成受精膜。这表明精子含有一种化学物质，可引发胞质钙增加，导致皮质颗粒胞吐。这也表明触发机制不需要外部激活的卵膜过程。进一步的实验表明，化学触发物不是钙。
