Vaughn Melonie, Powell Susan, Risbrough Victoria, Zhou Xianjin
Department of Psychiatry, University of California, San Diego, La Jolla, CA, United States of America.
VA Mental Illness Research and Clinical Core, San Diego, CA, United States of America.
PeerJ. 2025 Mar 31;13:e19212. doi: 10.7717/peerj.19212. eCollection 2025.
Low titers of blood circulating anti-NMDAR1 autoantibodies have been reported in a significant subset of the general human population. Currently, immunohistochemical staining and cell-based assays are the standard methods for their detection and semi-quantification. However, detection and quantification of these low titers of blood circulating anti-NMDAR1 autoantibodies are problematic because of high non-specific background. Development of a new method to more accurately quantify these low titers of blood anti-NMDAR1 autoantibodies will facilitate studies on their potential impacts on psychiatric symptoms and cognition. We previously reported a robust production of anti-NMDAR1 autoantibodies against the ligand binding domain of NMDAR1. As a proof of principle, we report the development of a novel simple immunoassay for quantification of cross-species blood anti-NMDAR1 autoantibodies and its validation with immunohistochemistry and cell-based assays in both humans and mice. Specificity of our quantification was also investigated.
据报道，在普通人群的一个重要亚组中存在低滴度的循环抗NMDAR1自身抗体。目前，免疫组织化学染色和基于细胞的检测方法是检测和半定量这些抗体的标准方法。然而，由于高非特异性背景，检测和定量这些低滴度的循环抗NMDAR1自身抗体存在问题。开发一种更准确地定量这些低滴度血液抗NMDAR1自身抗体的新方法将有助于研究它们对精神症状和认知的潜在影响。我们之前报道了针对NMDAR1配体结合域的抗NMDAR1自身抗体的强劲产生。作为原理证明，我们报告了一种用于定量跨物种血液抗NMDAR1自身抗体的新型简单免疫测定法的开发及其在人和小鼠中通过免疫组织化学和基于细胞的检测进行的验证。我们还研究了定量的特异性。
